PE Rat Anti-Mouse CD155

BD Pharmingen™ PE Rat Anti-Mouse CD155

Name: PE Rat Anti-Mouse CD155
Brand: BD Pharmingen™
SKU: 565797

Clone 3F1

(RUO)

Multicolor flow cytometric analysis of CD155 expression on mouse thymocytes. C57BL/6 mouse thymocytes were stained with APC Rat Anti-Mouse CD8a (Cat. No. 553035/561093) and BD Horizon™ BV421 Rat Anti-Mouse CD4 (Cat. No. 562891) antibodies, and either PE Rat IgG2a, κ Isotype Control (Cat. No. 553930; dashed line histograms) or PE Rat Anti-Mouse CD155 antibody (Cat. No. 565797; solid line histograms). The fluorescence histograms showing CD155 expression (or Ig Isotype control staining) were derived from CD4-CD8+ (Upper Left Plot), CD4+CD8+ (Upper Right), CD4-CD8- (Lower Left), or CD4+CD8- (Lower Right) gated events with the forward and side light-scatter characteristics of viable thymocytes. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.

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Product Details

Brand: BD Pharmingen™

Alternative Name: Poliovirus receptor; Pvr; Tumor-associated antigen 1; Taa1; Tage4; HVED

Reactivity: Mouse (QC Testing)

Isotype: Rat IgG2a, κ

Immunogen: Mouse CD155 Recombinant Protein

Application: Flow cytometry (Routinely Tested)

Concentration: 0.2 mg/ml

Entrez Gene ID: 52118

RRID: AB_2739361

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
An isotype control should be used at the same concentration as the antibody of interest.

Companion Products

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

Stain Buffer (BSA) RUO

Size 500 mL Cat No. 554657

PE Rat IgG2a, κ Isotype Control RUO

Size 0.1 mg Cat No. 553930

APC Rat Anti-Mouse CD8a RUO

Size 0.1 mg Cat No. 553035

APC Rat Anti-Mouse CD8a RUO

Size 25 µg Cat No. 561093

565797 Rev. 1

Antibody Details

3F1

The 3F1 monoclonal antibody specifically binds to CD155, which is also known as Poliovirus receptor (Pvr) or Tumor-associated antigen 1 (Taa1). CD155 is a type I transmembrane glycoprotein that belongs to the Ig supergene family. CD155 is an adhesion receptor that binds to different ligands including nectin-3, CD96, CD226, TIGIT, and the extracellular matrix protein vitronectin. It is highly expressed on double positive thymocytes and variably expressed on mature thymocytes and T cells, including regulatory T cells and NKT cells. CD155 is also differentially expressed on subsets of B cells, plasma cells, dendritic cells, and monocytes. CD155 expression is upregulated by activated T cells, B cells, and dendritic cells. CD155 is involved in forming adherens junctions between adjacent epithelial or endothelial cells. CD155 plays roles in regulating cell growth, adhesion, motility, migration, and cell-mediated cytotoxicity. CD155-deficient mice exhibit impaired secondary humoral immune responses to orally administered antigens.

565797 Rev. 1

Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

565797 Rev.1

Citations & References

Development References (3)

Danisch S, Qiu Q, Seth S, et al. CD226 interaction with CD155 impacts on retention and negative selection of CD8 positive thymocytes as well as T cell differentiation to follicular helper cells in Peyer's Patches.. Immunobiology. 2013; 218(2):152-8. (Biology). View Reference
Maier MK, Seth S, Czeloth N, et al. The adhesion receptor CD155 determines the magnitude of humoral immune responses against orally ingested antigens.. Eur J Immunol. 2007; 37(8):2214-25. (Immunogen: ELISA, Flow cytometry). View Reference
Stanietsky N, Rovis TL, Glasner A, et al. Mouse TIGIT inhibits NK-cell cytotoxicity upon interaction with PVR.. Eur J Immunol. 2013; 43(8):2138-50. (Clone-specific: Flow cytometry). View Reference

565797 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.