Skip to main content Skip to navigation
Hamburger Menu
Search icon
Order lookup icon Order lookup icon
Order Lookup
Country Selector Country Selector
United States (English)
Profile Icon Profile Icon
Sign-in/Register UserName
Products

Solutions

Discover & Learn

Resources & Tools

Support

Search icon Search icon
Close Menu
      Alert Icon
      Purified Mouse Anti-A2B5

      BD Pharmingen™ Purified Mouse Anti-A2B5

      Clone 105/A2B5 (also known as antibody A2B5, F12A2B5; 105)

      (RUO)
      View all Formats
      Purified Mouse Anti-A2B5
      Flow cytometric analysis of A2B5 expression on mouse pancreatic tumor (insulinoma) cells and Human embryonic stem cell (hESC)-derived neurons. Mouse Beta-TC-6 cells (ATCC, CRL-11506; left panel) or human H9 (WiCell, Madison, WI) hESC-derived neurons (right panel) were harvested with Accutase™ Cell Detachment Solution (Cat. No. 561527). The cells were stained with either Purified Mouse IgM, κ Isotype Control (Cat. No. 555581, dashed line histograms) or Purified Mouse Anti- A2B5 monoclonal antibody (solid line histograms) at matched concentrations. Dot plots were derived from gated events based on light scattering characteristics of viable cells. Flow cytometry was performed on a BD LSRFortessa™ flow cytometry system.
      Purified Mouse Anti-A2B5
      Flow cytometric analysis of A2B5 expression on mouse pancreatic tumor (insulinoma) cells and Human embryonic stem cell (hESC)-derived neurons. Mouse Beta-TC-6 cells (ATCC, CRL-11506; left panel) or human H9 (WiCell, Madison, WI) hESC-derived neurons (right panel) were harvested with Accutase™ Cell Detachment Solution (Cat. No. 561527). The cells were stained with either Purified Mouse IgM, κ Isotype Control (Cat. No. 555581, dashed line histograms) or Purified Mouse Anti- A2B5 monoclonal antibody (solid line histograms) at matched concentrations. Dot plots were derived from gated events based on light scattering characteristics of viable cells. Flow cytometry was performed on a BD LSRFortessa™ flow cytometry system.
      Product Details
      Down Arrow Up Arrow


      BD Pharmingen™
      Mouse (QC Testing), Human (Tested in Development), Rat, Chicken (Reported)
      Mouse BALB/c IgM
      Chicken embryo retina Cells
      Flow cytometry (Routinely Tested), Cytotoxicity (Reported), Bioimaging, Immunofluorescence (Not Recommended)
      0.5 mg/ml
      AB_2738421
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
      Show More blue down arrow Show Less blue up arrow

      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
      5. Accutase is a registered trademark of Innovative Cell Technologies, Inc.
      6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      Show More blue down arrow Show Less blue up arrow
      563775 Rev. 1
      Antibody Details
      Down Arrow Up Arrow
      105/A2B5

      The 105/A2B5 monoclonal antibody binds to gangliosides in neural tissue in the retina, brain, spinal cord, and dorsal root ganglia. The antigenic gangliosides are found on the plasma membrane of neural cell bodies, but not axons or dendrites. Expression levels of the gangliosides that mAb 105/A2B5 binds to, including GT3 and its O-acetylated derivative, decrease during embryonic development of the brain. Thus, mAb 105/A2B5 may be used to monitor the maturation of brain tissue. A2B5 antigens have also been associated with numerous tumors, including glioblastoma multiforme tumor cells, pancreatic islet tumor cells, and rat insulinoma cells, and are thought to contribute to chemoresistance and increased tumor proliferation and recurrence.

      563775 Rev. 1
      Format Details
      Down Arrow Up Arrow
      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      563775 Rev.1
      Citations & References
      Down Arrow Up Arrow

      Development References (2)

      1. Balik V, Mirossay P, Bohus P, Sulla I, Mirossay L, Sarissky M. Flow cytometry analysis of neural differentiation markers expression in human glioblastomas may predict their response to chemotherapy. 2009; 29:845-858. (Clone-specific: Flow cytometry). View Reference
      2. Eisenbarth GS, Walsh FS, Nirenberg M. Monoclonal antibody to a plasma membrane antigen of neurons. Proc Natl Acad Sci U S A. 1979; 76(10):4913-4917. (Immunogen: Cytotoxicity, Immunofluorescence). View Reference
      563775 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

      Website Feedback