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Flow cytometric analysis of Notch2 expression on human MCF-7 cells. Human MCF7 cells (ATCC Cat. No. HTB-22™; breast adenocarcinoma cell line) were harvested and stained with either PE Mouse IgG2a, κ Isotype Control (Cat. No. 554648; dashed line histogram) or PE Anti-Human Notch2 antibody (Cat. No. 563519; solid line histogram) at matched concentrations. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable MCF-7 cells. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometry System.
BD Pharmingen™ PE Mouse anti-Human Notch2
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Companion Products
The MHN2-25 monoclonal antibody specifically binds to an extracellular domain of human Notch2. Notch2 is a type 1 transmembrane glycoprotein receptor and member of the Notch family that includes Notch1-Notch4. Notch2 is cleaved in the Golgi and presents as a cell surface heterodimeric receptor. The Notch2 receptor can bind to several membrane-bound ligands including Jagged1, Jagged2, Delta1 and Delta4. Upon ligand binding, Notch2 undergoes proteolytic cleavage that results in the release of the Notch intracellular domain, NICD. NICD translocates to the nucleus where it forms a transcriptional activator complex with various transcriptional factors. These multimeric complexes either positively or negatively regulate the expression of multiple genes including those that orchestrate many facets of embryonic development and the subsequent functioning of multiple organ systems such as the hematopoietic, immune, cardiovascular, hepatic and renal systems. Notch2 is expressed by cells of the lung and brain, as well as cells of the B lineage, thymocytes, and T cells. Abnormalities in Notch2 expression may play a role in the development of B cell lymphomas.
Development References (4)
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Chiba S. Notch signaling in stem cell systems. Stem Cells. 2006; 24(11):2437-2447. (Biology). View Reference
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Haraguchi K, Suzuki T, Koyama N et al. Notch activation induces the generation of functional NK cells from human cord blood CD34-positive cells devoid of IL-15. J Immunol. 2009; 182(10):6168-6178. (Immunogen: Flow cytometry). View Reference
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Lammert E, Brown J, Melton DA. Notch gene expression during pancreatic organogenesis. 2000; 94(1-2):199-203. (Biology). View Reference
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Lee SY, Kumano K, Nakazaki K et al. Gain-of-function mutations and copy number increases of Notch2 in diffuse large B-cell lymphoma. 2009; 100(5):920-926. (Immunogen: ELISA). View Reference
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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