Flow cytometric analysis of CDX-2 expression on human DLD-1 cells. Cells from the human DLD-1 (Colorectal adenocarcinoma, ATCC CCL-221) cell line were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and washed with BD Pharmingen™ Stain Buffer (FBS) (Cat. No. 554656). The cells were then permeabilized and stained in BD™ Phosflow Perm/Wash Buffer I (Cat. No. 557885) with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; dashed line histogram) or PE Mouse Anti-CDX-2 antibody (Cat. No. 563428; solid line histogram). The fluorescence histograms were derived from gated events with the forward and side scattered-light characteristics of intact cells. This antibody also works with cells permeabilized with BD Cytofix/Cytoperm™ Fixation and Permeabilization Solution (Cat. No. 554722). Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
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Flow cytometric analysis of CDX-2 expression on human DLD-1 cells. Cells from the human DLD-1 (Colorectal adenocarcinoma, ATCC CCL-221) cell line were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and washed with BD Pharmingen™ Stain Buffer (FBS) (Cat. No. 554656). The cells were then permeabilized and stained in BD™ Phosflow Perm/Wash Buffer I (Cat. No. 557885) with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; dashed line histogram) or PE Mouse Anti-CDX-2 antibody (Cat. No. 563428; solid line histogram). The fluorescence histograms were derived from gated events with the forward and side scattered-light characteristics of intact cells. This antibody also works with cells permeabilized with BD Cytofix/Cytoperm™ Fixation and Permeabilization Solution (Cat. No. 554722). Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
Product Details
BD Pharmingen™
CDX2, CDX3, CDX-3
Human (QC Testing)
Mouse BALB/c IgG1, κ
Human CDX-2 Recombinant Protein
Intracellular staining (flow cytometry) (Routinely Tested)
5 µl
AB_2738198
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Fixation Buffer RUO
Size
100 mL
Cat No.
554655
Perm/Wash Buffer I RUO
Size
125 mL
Cat No.
557885
PE Mouse IgG1, κ Isotype Control RUO
Size
0.1 mg
Cat No.
554680
563428 Rev. 1
Antibody Details
M39-711
The M39-711 monoclonal antibody reacts with caudal-type homeobox transcription factor 2 (CDX-2). In embryonic development, CDX-2 is first expressed in the outer layer of the morula and later in the trophoblast, but not in embryonic stem cells. Along with Oct3/4, it is involved in the segregation of the trophoectoderm lineage from the inner cell mass. In the adult, CDX-2 expression is limited to the mucosa of the small and large intestines, where it regulates the synthesis of several intestine-specific proteins. Loss of CDX-2 expression is observed in some colorectal carcinomas, while gain of CDX-2 expression has been observed in gastric tumors and acute myeloid leukemias.
563428 Rev. 1
Format Details
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
563428 Rev.1
Citations & References
Development References (6)
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Hinoi T, Loda M, Fearon ER. Silencing of CDX2 expression in colon cancer via a dominant repression pathway. J Biol Chem. 2003; 278(45):44608-44616. (Biology). View Reference
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Qualtrough D, Hinoi T, Fearon E, Paraskeva C. Expression of CDX2 in normal and neoplastic human colon tissue and during differentiation of an in vitro model system. Gut. 2002; 51:184-190. (Biology). View Reference
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Ralston A, Rossant J. Genetic regulation of stem cell origins in the mouse embryo. Clin Genet. 2005; 68(2):106-112. (Biology). View Reference
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Rawat VP, Cusan M, Deshpande A, et al. Ectopic expression of the homeobox gene Cdx2 is the transforming event in a mouse model of (12;13)(p13;q12) acute myeloid leukemia. Proc Natl Acad Sci U S A. 2004; 101(3):817-822. (Biology). View Reference
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Scholl C, Bansal D, Döhner K, et al. The homeobox gene CDX2 is aberrantly expressed in most cases of acute myeloid leukemia and promotes leukemogenesis. J Clin Invest. 2007; 117(4):865-868. (Biology). View Reference
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Tsukamoto T, Mizoshita T, Tatematsu M. Gastric-and-intestinal mixed-type intestinal metaplasia: aberrant expression of transcription factors and stem cell intestinalization. Gastric Cancer. 2006; 9(3):156-166. (Biology). View Reference
563428 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
