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PE-CF594 Mouse anti-Mouse Siglec-G
PE-CF594 Mouse anti-Mouse Siglec-G
Multicolor flow cytometric analysis of Siglec-G expression on BALB/c splenocytes. Splenic leucocytes were stained with BD Horizon™ PE-CF594 Mouse anti-Mouse Siglec-G (Cat. No. 563337) and  Alexa Fluor® 700 Rat Anti-Mouse CD3 Molecular Complex (Cat. No. 561388) antibodies. A two-color flow cytometric dot plot showing the correlated expression patterns of Siglec-G versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes (Left Panel). Splenic leucocytes were similarly stained with Alexa Fluor® 647 Mouse anti-Mouse Siglec-G  and FITC Rat Anti-Mouse CD45R/B220 (Cat. No. 553088/553087/561877) antibodies. A two-color flow cytometric dot plot shows the correlated expression patterns of CD45R/B220 versus Siglec-G for viable lymphocytes (Right Panel). Flow cytometry was performed using a BD LSR™ II Flow Cytometer System.
Multicolor flow cytometric analysis of Siglec-G expression on BALB/c splenocytes. Splenic leucocytes were stained with BD Horizon™ PE-CF594 Mouse anti-Mouse Siglec-G (Cat. No. 563337) and  Alexa Fluor® 700 Rat Anti-Mouse CD3 Molecular Complex (Cat. No. 561388) antibodies. A two-color flow cytometric dot plot showing the correlated expression patterns of Siglec-G versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes (Left Panel). Splenic leucocytes were similarly stained with Alexa Fluor® 647 Mouse anti-Mouse Siglec-G  and FITC Rat Anti-Mouse CD45R/B220 (Cat. No. 553088/553087/561877) antibodies. A two-color flow cytometric dot plot shows the correlated expression patterns of CD45R/B220 versus Siglec-G for viable lymphocytes (Right Panel). Flow cytometry was performed using a BD LSR™ II Flow Cytometer System.
Product Details
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BD Horizon™
Siglecg; mSiglec-G; Siglec10; Siglec 10; CD330
Mouse (QC Testing)
Mouse IgG1, κ
Mouse Extracellular Siglec-G Recombinant Protein
Flow cytometry (Routinely Tested)
0.2 mg/ml
243958
AB_2738143
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon™ PE-CF594 under optimum conditions, and unconjugated antibody and free PE-CF594 were removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination.
  4. Because of the broad absorption spectrum of the tandem fluorochrome, extra care must be taken when using multi-laser cytometers, which may directly excite both PE and CF™594.
  5. When excited by the yellow-green (561-nm) laser, the fluorescence may be brighter than when excited by the blue (488-nm) laser.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  8. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  9. Texas Red is a registered trademark of Molecular Probes, Inc., Eugene, OR.
  10. CF™ is a trademark of Biotium, Inc.
  11. This product is provided under an Agreement between BIOTIUM and BD Biosciences. The manufacture, use, sale, offer for sale, or import of this product is subject to one or more patents or pending applications owned or licensed by Biotium, Inc. This product, and only in the amount purchased by buyer, may be used solely for buyer’s own internal research, in a manner consistent with the accompanying product literature. No other right to use, sell or otherwise transfer (a) this product, or (b) its components is hereby granted expressly, by implication or by estoppel. This product is for research use only. Diagnostic uses require a separate license from Biotium, Inc. For information on purchasing a license to this product including for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
  12. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
563337 Rev. 1
Antibody Details
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SH1

The SH1 monoclonal antibody specifically binds to mouse Siglec-G (sialic acid binding immunoglobulin-like lectin G), also known as Siglec10. Siglec-G is a Type I transmembrane glycoprotein that belongs to the Immunoglobulin superfamily. Siglec-G functions as an adhesion molecule that mediates sialic-acid dependent binding to cells. Siglec-G is expressed on cells of the B cell lineage. Siglec-G is most highly expressed by pre-B cells and B1a cells within the B cell lineage and is not detectable on T cells. Siglec-G can inhibit B cell receptor-mediated calcium signaling when it is overexpressed. Mice that lack Siglec-G have significantly increased numbers of B1a cells that begins early in development and is B cell intrinsic. Siglec-G-deficient mice have higher titers of natural IgM antibodies than their normal counterparts. Mouse Siglec-G is the ortholog of human Siglec-10 (also known as, CD330).

This antibody is conjugated to BD Horizon™ PE-CF594, which has been developed exclusively by BD Biosciences as a better alternative to PE-Texas Red®. PE-CF594 excites and emits at similar wavelengths to PE-Texas Red® yet exhibits improved brightness and spectral characteristics. Due to PE having maximal absorption peaks at 496 nm and 564 nm, PE-CF594 can be excited by the blue (488-nm), green (532-nm) and yellow-green (561-nm) lasers and can be detected with the same filter set as PE-Texas Red® (eg 610/20-nm filter).

563337 Rev. 1
Format Details
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PE-CF594
BD Horizon™ PE-CF594 dye is a part of the BD PE family of dyes. This tandem fluorochrome is comprised of a R-Phycoerythrin (PE) donor that has excitation maxima (Ex Max) of 496-nm and 566-nm and an acceptor dye with an emission maximum (Em Max) at 615-nm. PE-CF594, driven by BD innovation, is designed to be excited by the blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and detected using an optical filter centered near 615 nm (e.g., a 610/20-nm bandpass filter). The donor dye can be excited by the Blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and the acceptor dye can be excited by the green (532-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE-CF594
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
615 nm
563337 Rev.1
Citations & References
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Development References (4)

  1. Aizawa H, Zimmermann N, Carrigan PE, Lee JJ, Rothenberg ME, Bochner BS. Molecular analysis of human Siglec-8 orthologs relevant to mouse eosinophils: identification of mouse orthologs of Siglec-5 (mSiglec-F) and Siglec-10 (mSiglec-G). Genomics. 2003; 82(5):521-530. (Biology). View Reference
  2. Hoffmann A, Kerr S, Jellusova J, et al. Siglec-G is a B1 cell-inhibitory receptor that controls expansion and calcium signaling of the B1 cell population. Nat Immunol. 2007; 8(7):695-704. (Biology). View Reference
  3. Jellusova J, Düber S, Gückel E, et al. Siglec-G regulates B1 cell survival and selection. J Immunol. 2010; 185(6):3277-3284. (Biology). View Reference
  4. Nitschke L. CD22 and Siglec-G: B-cell inhibitory receptors with distinct functions. Immunol Rev. 2009; 230(1):128-143. (Biology). View Reference
View All (4) View Less
563337 Rev. 1

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.