BV711 Rat Anti-Mouse CD83

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BD Horizon™ BV711 Rat Anti-Mouse CD83

Name: BV711 Rat Anti-Mouse CD83
Brand: BD Horizon™
SKU: 563136

Clone Michel-19

(RUO)

Two-color flow cytometric analysis of CD83 expression by mature mouse dendritic cells. C57BL/6 bone marrow cells were cultured for 6 days with recombinant mouse GM-CSF (Cat. No. 554586) and stimulated for 24 hours with lipopolysaccharide. The bone marrow-derived dendritic cells were harvested and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with FITC Hamster Anti-Mouse CD11c antibody (Cat. No. 553801/557400/561045) and either BD Horizon™ BV711 Rat IgG1, κ Isotype Control (Cat. No. 563283; Left Panel) or BD Horizon™ BV711 Rat Anti-Mouse CD83 antibody (Cat. No. 563136; Right Panel). Two-color flow cytometric dot plots show the correlated expression patterns of CD11c versus CD83 (or Ig Isotype control staining) for gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.

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Product Details

Brand: BD Horizon™

Alternative Name: Cd83; CD83 antigen

Reactivity: Mouse (QC Testing)

Isotype: Rat IgG1, κ

Immunogen: Mouse CD83 Recombinant Protein

Application: Flow cytometry (Routinely Tested)

Concentration: 0.2 mg/ml

Entrez Gene ID: 12522

RRID: AB_2738024

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon™ BV711 under optimum conditions, and unconjugated antibody and free BD Horizon™ BV711 were removed.

Recommended Assay Procedures

For optimal and reproducible results, BD Horizon Brilliant™ Stain Buffer should be used anytime BD Horizon Brilliant™ dyes are used in a multicolor flow cytometry panel. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
An isotype control should be used at the same concentration as the antibody of interest.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
BD Horizon Brilliant Violet 711 is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,227,187; 8,455,613; 8,575,303; 8,354,239.
BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
Cy is a trademark of GE Healthcare.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

Companion Products

Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) RUO

Size 0.1 mg Cat No. 553141

Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) RUO

Size 0.5 mg Cat No. 553142

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

BV711 Rat IgG1, κ Isotype Control RUO

Size 50 µg Cat No. 563283

Recombinant Mouse GM-CSF RUO

Size 10 µg Cat No. 554586

563136 Rev. 2

Antibody Details

Michel-19

The Michel-19 antibody reacts with CD83, a member of the immunoglobulin superfamily that is expressed on mature dendritic cells and activated T lymphocytes. Furthermore, thymic cortical epithelial cells express Cd83 transcripts. CD83 is involved in the regulation of T-cell development and immune responses, and its ligand is found on a subpopulation of splenic B lymphocytes.

563136 Rev. 2

Format Details

BV711

The BD Horizon Brilliant Violet™ 711 (BV711) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This tandem fluorochrome is comprised of a BV421 donor with an excitation maximum (Ex Max) of 407-nm and an acceptor dye with an emission maximum (Em Max) at 713-nm. BV711, driven by BD innovation, is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 710-nm (e.g., a 712/20-nm bandpass filter). The acceptor dye can be excited by the Red (628–640-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: BV711

Excitation Source: Violet 405 nm

Excitation Max: 407 nm

Emission Max: 713 nm

563136 Rev.2

Citations & References

Development References (8)

Berchtold S, Muhl-Zurbes P, Heufler C, Winklehner P, Schuler G, Steinkasserer A. Cloning, recombinant expression and biochemical characterization of the murine CD83 molecule which is specifically upregulated during dendritic cell maturation. FEBS Lett. 1999; 461:211-216. (Biology). View Reference
Cramer SO, Trumpfheller C, Mehlhoop U, More S, Fleischer B, von Bonin A. Activation-induced expression of murine CD83 on T cells and identification of a specific CD83 ligand on murine B cells. Int Immunol. 2000; 12(9):1347-1351. (Biology). View Reference
Fujimoto Y, Tu L, Miller AS, et al. CD83 expression influences CD4+ T cell development in the thymus. Cell. 2002; 108:755-767. (Biology). View Reference
Kretschmer B, Kuhl S, Fleischer B, Breloer M. Activated T cells induce rapid CD83 expression on B cells by engagement of CD40. Immunol Lett. 2011; 136(2):221-227. (Clone-specific: Flow cytometry). View Reference
Kretschmer B, Luthje K, Ehrlich S, et al. CD83 on murine APC does not function as a costimulatory receptor for T cells. Immunol Lett. 2008; 120(1-2):87-95. (Immunogen: Flow cytometry). View Reference
Luthje K, Kretschmer B, Fleischer B, Breloer M. CD83 regulates splenic B cell maturation and peripheral B cell homeostasis. Int Immunol. 2008; 20(8):949-960. (Clone-specific: Flow cytometry). View Reference
Wolenski M, Cramer SO, Ehrlich S, Steeg C, Fleischer B, von Bonin A. Enhanced activation of CD83-positive T cells. Scand J Immunol. 2003; 58(3):306-311. (Biology). View Reference
Wolenski M, Cramer SO, Ehrlich S, et al. Expression of CD83 in the murine immune system. Med Microbiol Immunol (Berl). 2003; 192(4):189-192. (Biology). View Reference

View All (8)

563136 Rev. 2

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.