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      PE Mouse Anti-NeuroD1
      PE Mouse Anti-NeuroD1
      Flow cytometric analysis of NeuroD1 expression in human retinoblastoma. Y79 cells (ATCC, HTB-18™) were fixed with BD Cytofix™ fixation buffer (Cat. No. 554655) and permeabilized with BD Phosflow™ Perm buffer III (Cat. No. 558050). The cells were stained with either PE Mouse IgG1, κ isotype control (dashed line, Cat. No. 554680) or PE Mouse Anti-NeuroD1 monoclonal antibody (solid line) at matched concentrations.  Flow cytometry was performed on a BD LSRFortessa™ flow cytometry system.
      PE Mouse Anti-NeuroD1
      Flow cytometric analysis of NeuroD1 expression in human retinoblastoma. Y79 cells (ATCC, HTB-18™) were fixed with BD Cytofix™ fixation buffer (Cat. No. 554655) and permeabilized with BD Phosflow™ Perm buffer III (Cat. No. 558050). The cells were stained with either PE Mouse IgG1, κ isotype control (dashed line, Cat. No. 554680) or PE Mouse Anti-NeuroD1 monoclonal antibody (solid line) at matched concentrations.  Flow cytometry was performed on a BD LSRFortessa™ flow cytometry system.
      Product Details
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      BD Pharmingen™
      Class A basic helix-loop-helix protein 3, bHLHa3, BETA2, NeuroD
      Human (QC Testing), Mouse (Tested in Development)
      Mouse IgG1, κ
      Human NeuroD1 Recombinant Protein
      Intracellular staining (flow cytometry) (Routinely Tested)
      5 µl
      AB_2716791
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
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      Product Notices

      1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      2. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
      3. All other brands are trademarks of their respective owners.
      4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      563001 Rev. 1
      Antibody Details
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      R8-294

      The R8-294 monoclonal antibody specifically binds to Neurogenic differentiation factor 1 (NeuroD1).  NeuroD1 is a basic helix-loop-helix (bHLH) transcription factor that is also known as BHLHA3, Beta-cell E-box transactivator 2, BETA2 and NeuroD.  NeuroD1 interacts with other bHLH proteins and binds the insulin E-box sequence to regulate pancreatic islet cell development.  It also plays a key role in central nervous system (CNS) and sensory nervous system neuron development.   Specifically, it is required for the terminal differentiation of neurons during late stages of neurogenesis in the CNS (cerebral cortex, hippocampus, and cerebellum) and the sensory nervous system (eye, inner ear, and olfactory system).  During antibody development, the purified R8-294 monoclonal antibody was found to detect NeuroD1 by western blot and indirect immunofluorescent staining followed by flow cytometric analysis or imaging analysis.

      563001 Rev. 1
      Format Details
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      PE
      R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      PE
      Yellow-Green 488 nm, 532 nm, 561 nm
      496 nm, 566 nm
      576 nm
      563001 Rev.1
      Citations & References
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      Development References (9)

      1. Cherry TJ, Wang S, Bormuth I, Schwab M, Olson J, Cepko CL. NeuroD factors regulate cell fate and neurite stratification in the developing retina. J Neurosci. 2011; 31(20):7365-7379. (Biology). View Reference
      2. Cho JH, Tsai MJ. The role of BETA2/NeuroD1 in the development of the nervous system. Mol Neurobiol. 2004; 30(1):35-47. (Biology). View Reference
      3. Gao Z, Ure K, Ables JL, et al. Neurod1 is essential for the survival and maturation of adult-born neurons. Nat Neurosci. 2009; 12(9):1090-1092. (Biology). View Reference
      4. Habener JF, Kemp DM, Thomas MK. Minireview: transcriptional regulation in pancreatic development. Endocrinology. 2005; 146(3):1025-1034. (Biology). View Reference
      5. Kuwabara T, Hsieh J, Muotri A, et al. Wnt-mediated activation of NeuroD1 and retro-elements during adult neurogenesis. Nat Neurosci. 2009; 12(9):1097-10105. (Biology). View Reference
      6. Naya FJ, Huang HP, Qiu Y, et al. Diabetes, defective pancreatic morphogenesis, and abnormal enteroendocrine differentiation in BETA2/neuroD-deficient mice. Genes Dev. 1997; 11(18):2323-2334. (Biology). View Reference
      7. Naya FJ, Stellrecht CM, Tsai MJ. Tissue-specific regulation of the insulin gene by a novel basic helix-loop-helix transcription factor. Genes Dev. 1995; 9(8):1009-1019. (Biology). View Reference
      8. Pang ZP, Yang N, Vierbuchen T, et al. Induction of human neuronal cells by defined transcription factors. Nature. 2011; 476(7359):220-223. (Biology). View Reference
      9. Tamimi R, Steingrimsson E, Copeland NG, et al. The NEUROD gene maps to human chromosome 2q32 and mouse chromosome 2. Genomics. 1996; 15(34):418-421. (Biology). View Reference
      View All (9) View Less
      563001 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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