PE-CF594 Mouse Anti-Human CD147

BD Horizon™ PE-CF594 Mouse Anti-Human CD147

Name: PE-CF594 Mouse Anti-Human CD147
Brand: BD Horizon™
SKU: 562553

Clone HIM6

(RUO)

Flow cytometric analysis of CD147 expression on human peripheral blood lymphocytes. Whole blood was stained with BD Horizon™ PE-CF594 Mouse Anti-Human CD147 antibody (Cat. No. 562553; solid line histogram) or with a BD Horizon™ PE-CF594 Mouse IgG1, κ Isotype Control (Cat. No. 562292; dashed line histogram). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No.555899). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.

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Product Details

Brand: BD Horizon™

Alternative Name: BSG; Basigin; BASI; Neurothelin; 5F7; EMMPRIN; M6; OK; TCSF

Reactivity: Human (QC Testing)

Isotype: Mouse IgG1, κ

Application: Flow cytometry (Routinely Tested)

Vol. Per Test: 5 µl

Workshop Number: VI NL109

RRID: AB_2737649

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
An isotype control should be used at the same concentration as the antibody of interest.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Texas Red is a registered trademark of Molecular Probes, Inc., Eugene, OR.
CF™ is a trademark of Biotium, Inc.
When excited by the yellow-green (561-nm) laser, the fluorescence may be brighter than when excited by the blue (488-nm) laser.
This product is provided under an Agreement between BIOTIUM and BD Biosciences. The manufacture, use, sale, offer for sale, or import of this product is subject to one or more patents or pending applications owned or licensed by Biotium, Inc. This product, and only in the amount purchased by buyer, may be used solely for buyer’s own internal research, in a manner consistent with the accompanying product literature. No other right to use, sell or otherwise transfer (a) this product, or (b) its components is hereby granted expressly, by implication or by estoppel. This product is for research use only. Diagnostic uses require a separate license from Biotium, Inc. For information on purchasing a license to this product including for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
Because of the broad absorption spectrum of the tandem fluorochrome, extra care must be taken when using multi-laser cytometers, which may directly excite both PE and CF™594.
Please refer to http://regdocs.bd.com to access safety data sheets (SDS).

Companion Products

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

Lysing Buffer RUO

Size 100 mL Cat No. 555899

PE-CF594 Mouse IgG1, κ Isotype Control RUO

Size 0.1 mg Cat No. 562292

562553 Rev. 2

Antibody Details

HIM6

The HIM6 monoclonal antibody specifically binds to CD147 which is encoded by BSG. CD147 is a type I transmembrane glycoprotein (30-50 kDa) of the immunoglobulin super-gene family. Neurothelin, a blood-brain barrier-specific molecule, was clustered as CD147 in the Sixth Human Leukocyte Differentiation Antigen (HLDA) workshop. It bears homology with mouse gp42 or basigin, human "M6" or "EMMPRIN", rat OX-47 or CD-9, and avian HT7 or 5A11. CD147 is also known as Tumor cell-derived collagenase stimulatory factor (TCSF). CD147 is a molecule that is broadly expressed on cells of hematopoietic and non-hematopoietic origin. Its expression on specific cell types may be regulated by cytokines. CD147 plays a role in embryonal blood-brain barrier development and a role in integrin-mediated adhesion in brain endothelia.

This antibody is conjugated to BD Horizon™ PE-CF594, which has been developed exclusively by BD Biosciences as a better alternative to PE-Texas Red®. PE-CF594 excites and emits at similar wavelengths to PE-Texas Red® yet exhibits improved brightness and spectral characteristics. Due to PE having maximal absorption peaks at 496 nm and 564 nm, PE-CF594 can be excited by the blue (488-nm), green (532-nm) and yellow-green (561-nm) lasers and can be detected with the same filter set as PE-Texas Red® (eg, 610/20-nm filter).

562553 Rev. 2

Format Details

PE-CF594

BD Horizon™ PE-CF594 dye is a part of the BD PE family of dyes. This tandem fluorochrome is comprised of a R-Phycoerythrin (PE) donor that has excitation maxima (Ex Max) of 496-nm and 566-nm and an acceptor dye with an emission maximum (Em Max) at 615-nm. PE-CF594, driven by BD innovation, is designed to be excited by the blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and detected using an optical filter centered near 615 nm (e.g., a 610/20-nm bandpass filter). The donor dye can be excited by the Blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and the acceptor dye can be excited by the green (532-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE-CF594

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 615 nm

562553 Rev.2

Citations & References

Development References (5)

Majdic O, Pickl WF, Kohl P, Stockinger H, Knapp W. EC16.3 CD147 Workshop: Reactivity and epitope mapping of CD147 monoclonal antibodies. In: Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997:765-766.
Riethdorf S, Reimers N, Assmann V, et al. High incidence of EMMPRIN expression in human tumors. Int J Cancer. 2006; 119(8):1800-1810. (Clone-specific: Immunohistochemistry, Immunoprecipitation, Western blot). View Reference
Rizzo A. Aragona E, Dino O, et al. EC16.1 CD147 Workshop: Expression of CD147 (neurothelin) in liver and lung cancer. In: Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997:763-764.
Stockinger H, Ebel T, Hansmann C, et al.. EC16 CD147 (neurothelin/basigin) Workshop Panel Report. In: Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997:760-763.
Sudou A, Ozawa M, Muramatsu T. Lewis X structure increases cell substratum adhesion in L cells. J Biochem (Tokyo). 1995; 117(2):271-275. (Biology). View Reference

View All (5)

562553 Rev. 2

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.