V450 Mouse Anti-Rat CD11b

BD Horizon™ V450 Mouse Anti-Rat CD11b

Name: V450 Mouse Anti-Rat CD11b
Brand: BD Horizon™
SKU: 562108

Clone WT.5

(RUO)

Multiparameter flow cytometric analysis of CD11b expression on rat bone marrow. Lewis rat bone marrow cells were stained with either BD Horizon™ V450 Mouse IgA, κ Isotype Control (Cat. No. 562142, Left Panel) or a BD Horizon™ V450 Mouse Anti-Rat CD11b antibody (Cat. No. 562108, Right Panel). Two parameter flow cytometric dot blots showing side light scatter signals (SSC-A) versus CD11b expression (or Ig Isotype Control staining) were derived from gated events with the forward light-scatter characteristics of viable bone marrow cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.

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Product Details

Brand: BD Horizon™

Alternative Name: Itgam; Integrin αM chain; Mac-1 α chain; Integrin beta 2 alpha subunit

Reactivity: Rat (QC Testing)

Isotype: Mouse BALB/c IgA, κ

Immunogen: Rat neutrophils

Application: Flow cytometry (Routinely Tested)

Concentration: 0.2 mg/ml

RRID: AB_10898164

Storage Buffer: Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon™ V450 under optimum conditions, and unreacted BD Horizon™ V450 was removed.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
An isotype control should be used at the same concentration as the antibody of interest.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
BD Horizon V450 has a maximum absorption of 406 nm and maximum emission of 450 nm. Before staining with this reagent, please confirm that your flow cytometer is capable of exciting the fluorochrome and discriminating the resulting fluorescence.
Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.

Companion Products

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

V450 Mouse IgA, κ Isotype Control RUO

Size 0.1 mg Cat No. 562142

562108 Rev. 1

Antibody Details

WT.5

The WT.5 monoclonal antibody specifically binds to CD11b, the α subunit of Mac-1 found on neutrophils and myeloid cells, but not on lymphocytes. Mac-1 expression is rapidly upregulated on neutrophils after activation, in the same time period that CD62L (L-selectin) is shed from the cell surface. Mac-1 mediates adhesion to ICAM-1 (CD54) and C3bi. Neutrophil binding to ICAM-1 is blocked by mAb WT.5.

The antibody is conjugated to BD Horizon™ V450, which has been developed for use in multicolor flow cytometry experiments and is available exclusively from BD Biosciences. It is excited by the Violet laser Ex max of 406 nm and has an Em Max at 450 nm. Conjugates with BD Horizon™ V450 can be used in place of Pacific Blue™ conjugates.

562108 Rev. 1

Format Details

V450

BD Horizon™ V450 Dye is part of the BD Horizon™ violet family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 405-nm and an emission maximum (Em Max) at 450-nm. BD Horizon™ V450, driven by BD innovation, is designed to be excited by the violet laser (405 nm) and detected using an optical filter centered near 450-nm (e.g., a 450/50-nm bandpass filter). The dye can be excited by the UV (355-nm) laser resulting in cross-laser excitation and spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: V450

Excitation Source: Violet 405 nm

Excitation Max: 405 nm

Emission Max: 450 nm

562108 Rev.1

Citations & References

Development References (1)

Tamatani T, Kitamura F, Kuida K, et al. Characterization of rat LECAM-1 (L-selectin) by the use of monoclonal antibodies and evidence for the presence of soluble LECAM-1 in rat sera. Eur J Immunol. 1993; 23(9):2181-2188. (Immunogen). View Reference

562108 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.