Western Blot analysis of Sox2 in Human Neural Stem Cells (NSC) and Mouse Embryonic Stem (ES) cell line. Lysates of NSC derived from H9 human ES cells (WiCell, Madison, WI, Left) and ES-E14TG2a mouse ES cells (ATCC CRL-1821, right) were probed with Purified Mouse anti-Sox2 monoclonal antibody at concentrations of 0.125 (lanes 1), 0.06 (lanes 2), and 0.03 μg/ml (lanes 3). Human and mouse Sox2 were identified as bands of ~36 kDa.
Immunoflourescent staining of Sox2 in human embryonic stem (ES) cells. H9 human ES cells (WiCell Madison, WI), passage 52 grown in mTESR™1 medium (StemCell Technologies) on BD Matrigel™ hESC-qualified Matrix (Cat. No. 354277), were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655), permeabilized with BD™ Phosflow Perm Buffer III (Cat No. 558050), and stained with Purified Mouse anti-Sox2 monoclonal antibody (pseudo colored green) at 10 μg/mL. The second step reagent was Alexa Fluor® 488 goat anti-mouse Ig (Life Technologies), and counter-staining was with Hoechst 33342 (pseudo-colored blue). The images were captured on a BD Pathway™ 435 Cell Analyzer and merged using BD Attovision™ Software. Permeabilization with BD Perm/Wash™ (Cat No. 554723) or 0.1% Triton™ X-100 buffer is also suitable for use with this antibody.
Western Blot analysis of Sox2 in Human Neural Stem Cells (NSC) and Mouse Embryonic Stem (ES) cell line. Lysates of NSC derived from H9 human ES cells (WiCell, Madison, WI, Left) and ES-E14TG2a mouse ES cells (ATCC CRL-1821, right) were probed with Purified Mouse anti-Sox2 monoclonal antibody at concentrations of 0.125 (lanes 1), 0.06 (lanes 2), and 0.03 μg/ml (lanes 3). Human and mouse Sox2 were identified as bands of ~36 kDa.
Immunoflourescent staining of Sox2 in human embryonic stem (ES) cells. H9 human ES cells (WiCell Madison, WI), passage 52 grown in mTESR™1 medium (StemCell Technologies) on BD Matrigel™ hESC-qualified Matrix (Cat. No. 354277), were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655), permeabilized with BD™ Phosflow Perm Buffer III (Cat No. 558050), and stained with Purified Mouse anti-Sox2 monoclonal antibody (pseudo colored green) at 10 μg/mL. The second step reagent was Alexa Fluor® 488 goat anti-mouse Ig (Life Technologies), and counter-staining was with Hoechst 33342 (pseudo-colored blue). The images were captured on a BD Pathway™ 435 Cell Analyzer and merged using BD Attovision™ Software. Permeabilization with BD Perm/Wash™ (Cat No. 554723) or 0.1% Triton™ X-100 buffer is also suitable for use with this antibody.
Product Details
BD Pharmingen™
Human (QC Testing), Mouse (Reactivity Confirmed in Development)
Mouse CD, also known as Charles River SD (outbred) IgG1, κ
Human Sox2 Recombinant Protein
Western blot (Routinely Tested), Bioimaging, Immunofluorescence, Intracellular staining (flow cytometry) (Tested During Development)
36 kDa
0.5 mg/ml
6657, 20674
AB_10694256
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Product Notices
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- mTESR™1 is a trademark of StemCell Technologies.
- Triton is a trademark of the Dow Chemical Company.
- Alexa Fluor® is a registered trademark of Life Technologies Corporation.
Companion Products
Fixation Buffer RUO
Size
100 mL
Cat No.
554655
Perm Buffer III RUO
Size
125 mL
Cat No.
558050
Perm/Wash Buffer RUO
Size
100 mL
Cat No.
554723
HRP Goat Anti-Mouse Ig RUO
Size
1 mL
Cat No.
554002
561469 Rev. 1
Antibody Details
O30-678
The monoclonal antibody O30-678 recognizes the Sox2 transcription factor. Sox2 [SRY (sex determining region Y)-box 2] is a member of the SRY-related HMG-box (SOX) family of transcription factors. Sox2 is required for the maintenance of the undifferentiated state of pluripotent stem cells. Complexes of Sox2 with the homeobox transcription factors Oct3/4 and/or Nanog bind to the promoters of a network of genes that are involved in the maintenance of pluripotency and self renewal in stem cells. Sox2 is also a marker of neural stem cells during embryonic development and in the adult brain. The O30-678 antibody recognizes both human and mouse Sox2 proteins.
561469 Rev. 1
Format Details
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
561469 Rev.1
Citations & References
Development References (3)
-
Boyer LA, Lee TI, Cole MF, et al. Core transcriptional regulatory circuitry in human embryonic stem cells. Cell. 2005; 122:947-956. (Biology). View Reference
-
Pan G, Thomson JA. Nanog and transcriptional networks in embryonic stem cell pluripotency. Cell Res. 2007; 17:42-49. (Biology). View Reference
-
Takahashi K, Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell. 2006; 126(4):663-676. (Biology). View Reference
561469 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
