Skip to main content Skip to navigation
Hamburger Menu
Search icon
Order lookup icon Order lookup icon
Order Lookup
Country Selector Country Selector
United States (English)
Profile Icon Profile Icon
Sign-in/Register UserName
Products

Solutions

Discover & Learn

Resources & Tools

Support

Search icon Search icon
Close Menu
      Alert Icon
      Purified Mouse Anti-c-Cbl (pY774)
      Purified Mouse Anti-c-Cbl (pY774)
      Western blot analysis of c-Cbl (pY774) in human T leukemia.  Lysates from control (Cat. No. 611451, left panel) and Pervanadate-treated (Cat. No. 611755, right panel) Jurkat cells were probed with purified mouse anti-c-Cbl (pY774) monoclonal antibody at concentrations of 1.0, 0.5, and 0.25 µg/ml (Lanes 1, 2, and 3, respectively).  c-Cbl (pY774) is identified as a band of 120 kDa in the treated cells.
      Purified Mouse Anti-c-Cbl (pY774)
      c-Cbl (pY774) staining on tonsil.  Fresh human tonsil was incubated in 5 mM Pervanadate solution for 2 hours, then fixed in formalin and processed. Following antigen retrieval with BD Retrievagen A buffer (Cat. no. 550524), the sections were either left untreated (left panel) or treated with a phosphatase to eliminate all phosphorylation (right panel).  The tissue sections were stained with purified mouse anti-c-Cbl (pY774) with Hematoxylin counterstaining. Original magnification: 20X.
      Purified Mouse Anti-c-Cbl (pY774) Purified Mouse Anti-c-Cbl (pY774)
      Western blot analysis of c-Cbl (pY774) in human T leukemia.  Lysates from control (Cat. No. 611451, left panel) and Pervanadate-treated (Cat. No. 611755, right panel) Jurkat cells were probed with purified mouse anti-c-Cbl (pY774) monoclonal antibody at concentrations of 1.0, 0.5, and 0.25 µg/ml (Lanes 1, 2, and 3, respectively).  c-Cbl (pY774) is identified as a band of 120 kDa in the treated cells.
      c-Cbl (pY774) staining on tonsil.  Fresh human tonsil was incubated in 5 mM Pervanadate solution for 2 hours, then fixed in formalin and processed. Following antigen retrieval with BD Retrievagen A buffer (Cat. no. 550524), the sections were either left untreated (left panel) or treated with a phosphatase to eliminate all phosphorylation (right panel).  The tissue sections were stained with purified mouse anti-c-Cbl (pY774) with Hematoxylin counterstaining. Original magnification: 20X.
      Product Details
      Down Arrow Up Arrow


      BD Pharmingen™
      Human (QC Testing)
      Mouse IgG1, κ
      Phosphorylated Human c-Cbl Peptide
      Western blot (Routinely Tested), Immunohistochemistry-formalin (antigen retrieval required) (Tested During Development)
      0.5 mg/ml
      AB_397000
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
      Show More blue down arrow Show Less blue up arrow

      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      Show More blue down arrow Show Less blue up arrow
      558035 Rev. 2
      Antibody Details
      Down Arrow Up Arrow
      29/c-Cbl

      Cbl (Casitas B-lineage lymphoma) was identified in the genome of a transforming retrovirus from a mouse pre-B lymphoma.  The cellular gene product c-Cbl is one of numerous Cbl-related proteins found in vertebrate and invertebrate organisms.  It is an 120-kDa adapter protein that contains multiple functional domains, including a RING finger motif, a tyrosine kinase-binding (TKB) domain, and a proline-rich region.  The TKB domain directly interacts with specific auto-phosphorylation sites in activated protein-tyrosine kinases (PTK).  Through the RING finger motif, c-Cbl recruits and activates an E2 ubiquitin-conjugating enzyme, thus targeting the activated PTK for protein degradation.  The proline-rich region contains SH3 domain-binding and 14-3-3 protein-binding motifs.  c-Cbl is also phosphorylated at tyrosines 700, 731, and 774 (Y774) by Syk- and Src-family kinases after the stimulation of some integrins and a wide variety of receptors for antigens, immunoglobulins, growth factors, cytokines, and hormones.  In turn, the phosphorylated Y774 site interacts with the SH2 domain of the CRK adapter protein.  The c-Cbl adapter protein is expressed in the cytoplasm in all tissues, with especially high levels of expression in hematopoietic cells.  Through its many functional sites, c-Cbl plays key roles in the positive and negative regulation of vital cell functions, including T Cell Receptor-mediated cellular immune responses.

      The 29/c-Cbl monoclonal antibody recognizes the Y774-phosphorylated form of human c-Cbl.

      558035 Rev. 2
      Format Details
      Down Arrow Up Arrow
      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      558035 Rev.2
      Citations & References
      Down Arrow Up Arrow

      Development References (2)

      1. Thien CBF, Langdon WY. CBL: Many adaptations to regulate protein tyrosine kinase. Nat Rev Mol Cell Biol. 2001; 2:294-307. (Biology).
      2. Tsygankov AY, Teckchandani AM, Feshchenko EA, Swaminathan G. Beyond the RING: CBL proteins as multivalent adapters. Oncogene. 2001; 20:6382-6402. (Biology).
      558035 Rev. 2

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

      Website Feedback