Preparation And Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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The 6D4 monoclonal antibody specifically binds to the human MHC class I polypeptide-related sequence A (MICA, aka PERB11.1) and B (MICB. aka PERB11.2) proteins. These ~70 kDa transmembrane glycoproteins are homologs of the major histocompatibility complex class I molecules although they lack association with β2 microglobulin. The MHC class I-related MICA and MICB chains are expressed by some gut epithelial cells in vivo. MICA and MICB expression by other epithelial cells and cell types, including fibroblasts and endothelial cells, is induced by stress, eg, stress caused by bacterial and viral infections, autoimmunity or cellular transformation. Epithelial cell expression of MICA and MICB has also been detected in transplanted kidneys and pancreas that show histological signs of rejection and or cellular injury. This suggests their potential role in transplant immunopathology. MICA and MICB are ligands for NKG2D (CD314), an activating receptor expressed by natural killer (NK) cells, γδ T cells, CD8+ and some CD4+ αβ T cells. The 6D4 antibody reportedly blocks NKG2D-positive NK cell- and T cell-mediated cytotoxicity against MICA/B-positive target cells.
Development References (7)
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Bauer S, Groh V, Wu J, et al. Activation of NK cells and T cells by NKG2D, a receptor for stress-inducible MICA. Science. 1999; 285(5428):727-729. (Biology). View Reference
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Das H, Groh V, Kuijl C, et al. MICA engagement by human Vgamma2Vdelta2 T cells enhances their antigen-dependent effector function. Immunity. 2001; 15(1):83-93. (Biology). View Reference
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Groh V, Bahram S, Bauer S, Herman A, Beauchamp M, Spies T. Cell stress-regulated human major histocompatibility complex class I gene expressed in gastrointestinal epithelium. Proc Natl Acad Sci U S A. 1996; 93(22):12445-12450. (Biology). View Reference
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Groh V, Bruhl A, El-Gabalawy H, Nelson JL, Spies T. Stimulation of T cell autoreactivity by anomalous expression of NKG2D and its MIC ligands in rheumatoid arthritis. Proc Natl Acad Sci U S A. 2003; 100(16):9452-9457. (Biology). View Reference
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Groh V, Rhinehart R, Randolph-Habecker J, Topp MS, Riddell SR, Spies T. Costimulation of CD8alphabeta T cells by NKG2D via engagement by MIC induced on virus-infected cells. Nat Immunol. 2001; 2(3):255-260. (Biology). View Reference
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Groh V, Rhinehart R, Secrist H, Bauer S, Grabstein KH, Spies T. Broad tumor-associated expression and recognition by tumor-derived gamma delta T cells of MICA and MICB. Proc Natl Acad Sci U S A. 1999; 96(12):6879-6884. (Biology). View Reference
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Hankey KG, Drachenberg CB, Papadimitriou JC, et al. MIC expression in renal and pancreatic allografts. Transplantation. 2002; 73(2):304-306. (Biology). View Reference
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
