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Purified Mouse Anti-Human CD206
Purified Mouse Anti-Human CD206
Flow cytometric analysis of CD206 expression by GM-CSF-stimulated macrophages.  Human peripheral blood mononuclear cells were cultured for 3 days with Recombinant Human GM-CSF (Cat. No. 550068). The cells were harvested and stained with either Purified Mouse IgG1, κ Isotype Control (Cat. No. 555746; dashed line histogram) or Purified Mouse Anti-Human CD206 antibody (Cat. No. 555953; solid line histogram). CD206 (or Ig isotype control) expression was visualized with FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555988). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable GM-CSF activated monocytes. Flow cytometric analysis was performed using a BD FACScan system.
Flow cytometric analysis of CD206 expression by GM-CSF-stimulated macrophages.  Human peripheral blood mononuclear cells were cultured for 3 days with Recombinant Human GM-CSF (Cat. No. 550068). The cells were harvested and stained with either Purified Mouse IgG1, κ Isotype Control (Cat. No. 555746; dashed line histogram) or Purified Mouse Anti-Human CD206 antibody (Cat. No. 555953; solid line histogram). CD206 (or Ig isotype control) expression was visualized with FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555988). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable GM-CSF activated monocytes. Flow cytometric analysis was performed using a BD FACScan system.
Product Details
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BD Pharmingen™
Macrophage mannose receptor; MMR; MRC1; CLEC13D
Human (QC Testing)
Mouse IgG1, κ
Human Placental Macrophage Mannose Receptor
Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development)
0.5 mg/ml
VII 70318
AB_396249
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
555953 Rev. 8
Antibody Details
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19.2

The 19.2 monoclonal antibody specifically binds to CD206 which is also known as the macrophage mannose receptor (MMR) or C-type lectin domain family 13 member D (CLEC13D). CD206 is a type I transmembrane glycoprotein of approximately 162 kDa which binds to glycoconjugates containing mannose, fucose, or N-acetylglucosamine. These carbohydrates are present on the surface of many microorganisms and enable the macrophage to bind to microorganisms through the MMR and internalize them during the process of phagocytosis. CD206 is expressed on human macrophages, endothelial cells, and cultured dendritic cells. It is not detected on resting monocytes. Mannose receptor expression is upregulated on peripheral blood mononuclear cells following 3 day incubation with GM-CSF.

555953 Rev. 8
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
555953 Rev.8
Citations & References
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Development References (3)

  1. Hbid O, Belloul L, Fajali N, et al. Kaposi's sarcoma in Morocco: a pathological study with immunostaining for human herpesvirus-8 LNA-1.. Pathology. 2005; 37(4):288-95. (Clone-specific). View Reference
  2. Mason D. David Mason .. et al., ed. Leucocyte typing VII : white cell differentiation antigens : proceedings of the Seventh International Workshop and Conference held in Harrogate, United Kingdom. Oxford: Oxford University Press; 2002.
  3. Pontow SE, Kery V, Stahl PD. Mannose receptor. Int Rev Cytol. 1992; 137(B):221-244. (Clone-specific). View Reference
555953 Rev. 8

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.