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      FITC Mouse Anti-Human CD46
      FITC Mouse Anti-Human CD46
      Flow cytometric analysis of CD46 expression on human peripheral blood lymphocytes. Whole blood was stained with FITC Mouse Anti-Human CD46 (Cat. No. 555949; solid line histogram) and FITC Mouse IgG2a, κ Isotype Control (Cat. No.555573, dashed line histogram). Erythrocytes were lysed with Lysing Buffer (Cat. No. 555899). The fluorescence histogram was derived from gated events with the forward and side light-scattering characteristics of viable lymphocytes.
      FITC Mouse Anti-Human CD46
      Flow cytometric analysis of CD46 expression on human peripheral blood lymphocytes. Whole blood was stained with FITC Mouse Anti-Human CD46 (Cat. No. 555949; solid line histogram) and FITC Mouse IgG2a, κ Isotype Control (Cat. No.555573, dashed line histogram). Erythrocytes were lysed with Lysing Buffer (Cat. No. 555899). The fluorescence histogram was derived from gated events with the forward and side light-scattering characteristics of viable lymphocytes.
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      BD Pharmingen™
      AHUS2; Membrane cofactor protein; MCP; MIC10; TLX; TRA2.10
      Human (QC Testing)
      Mouse IgG2a, κ
      Flow cytometry (Routinely Tested)
      20 µl
      IV N24
      4179
      AB_396245
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
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      Product Notices

      1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      555949 Rev. 7
      Antibody Details
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      E4.3

      The E4.3 monoclonal antibody specifically binds to CD46. CD46 is also known as membrane cofactor protein (MCP). CD46 is a type I membrane glycoprotein composed of two non-disulfide linked α (66 kDa) and β (56 kDa) chains expressed on lymphocytes, monocytes and granulocytes. It is not expressed on erythrocytes or platelets. There are four isoforms of the dimer which function as complement regulatory factors. CD46 promotes the enzymatic degradation of activated C3b and/or C4b deposited on host cells. It also serves as the measles virus receptor.

      555949 Rev. 7
      Format Details
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      FITC
      Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      FITC
      Blue 488 nm
      494 nm
      518 nm
      555949 Rev.7
      Citations & References
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      Development References (3)

      1. Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182.
      2. Kurita M, Yanagi Y, Hara T, Nagasawa S, Matsumoto M, Seya T. Human lymphocytes are more susceptible to measles virus than granulocytes, which is attributable to the phenotypic differences of their membrane cofactor protein (CD46). Immunol Lett. 1995; 48(2):91-95. (Biology). View Reference
      3. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
      555949 Rev. 7

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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