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      Purified Mouse Anti-Human CD74
      Purified Mouse Anti-Human CD74
      Flow cytometric analysis of CD74 expression on human peripheral lymphocytes. Human whole blood was stained with either Purified Mouse Anti-Human CD74 (Cat. No. 555612; solid line histogram) or Purified Mouse IgG1, κ Isotype Control (Cat. No. 555746; dashed line histogram), followed by FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555988). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Fluorescent histograms were derived from gated events with the forward and side light-scattering characteristic of viable lymphocytes. Flow cytometry was performed on a BD FACScan™ system.
      Purified Mouse Anti-Human CD74
      Flow cytometric analysis of CD74 expression on human peripheral lymphocytes. Human whole blood was stained with either Purified Mouse Anti-Human CD74 (Cat. No. 555612; solid line histogram) or Purified Mouse IgG1, κ Isotype Control (Cat. No. 555746; dashed line histogram), followed by FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555988). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Fluorescent histograms were derived from gated events with the forward and side light-scattering characteristic of viable lymphocytes. Flow cytometry was performed on a BD FACScan™ system.
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      BD Pharmingen™
      DHLAG; HLADG; HLA-DR-gamma; HG2A; Ia-GAMMA
      Human (QC Testing)
      Mouse BALB/c IgG1, κ
      Nuclei from Human SU-DHL-4 Lymphoma Cell Line
      Flow cytometry (Routinely Tested), Immunohistochemistry-formalin (antigen retrieval required), Immunohistochemistry-frozen (Tested During Development)
      0.5 mg/ml
      IV B129; V CD74.3
      AB_395979
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
      5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      555612 Rev. 8
      Antibody Details
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      LN2

      The LN2 monoclonal antibody specifically recognizes CD74, the cell surface MHC class II-associated invariant chain molecule (41, 35, 33 kDa). CD74 is moderately expressed on B cells, and weakly on monocytes and macrophages. Other reactive cells include Langerhans cells, dendritic cells, a subset of activated T cells and thymic epithelium. The LN2 monoclonal antibody labels a nuclear membrane protein of follicular center and mantle zone B cells and a cytoplasmic protein of cells which bind HLA class II antibodies. CD74 is postulated to play a role in antigen processing and presentation through intracellular transport and expression of class II molecules on the cell surface. This antibody is suitable for staining formalin-fixed, paraffin-embedded tissue sections without citrate pretreatment.

      555612 Rev. 8
      Format Details
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      555612 Rev.8
      Citations & References
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      Development References (4)

      1. Dorken B, Moller P, Pezzutto A, Schwartz-Albiez R, Moldenhaur G.. B-cell antigens: CD74. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:106-108.
      2. Epstein AL, Marder RJ, Winter JN, Fox RI. Two new monoclonal antibodies (LN-1, LN-2) reactive in B5 formalin-fixed, paraffin-embedded tissues with follicular center and mantle zone human B lymphocytes and derived tumors.. J Immunol. 1984; 133(2):1028-36. (Immunogen). View Reference
      3. Moller P, Henne C, Moldenhauer G. CD74 Workshop Panel report. In: Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995:568-571.
      4. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
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      555612 Rev. 8

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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