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      Purified Rat Anti-Mouse I-A/I-E
      Product Details
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      BD Pharmingen™
      H-2I; I-Ad, -Ed; I-Eb and I-Ek MHC class II alloantigens; Ia Ag; MHC II
      Mouse (QC Testing)
      Rat DA, also known as DA/HA IgG2a, κ
      BALB/c mouse epidermal Langerhans cells
      Flow cytometry (Routinely Tested), Immunoprecipitation, Intracellular staining (flow cytometry) (Reported)
      0.5 mg/ml
      14961, 14969
      AB_394956
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
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      Recommended Assay Procedures

      Other reported applications include immunoprecipitation and intracellular immunofluorescent staining.

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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      553621 Rev. 12
      Antibody Details
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      2G9

      The 2G9 monoclonal antibody reacts with the I-Ad and I-Ed MHC class II alloantigens.  It also reacts with transfectants expressing I-Eb and I-Ek and with cells from mice of the H-2p and H-2q haplotypes.  This antibody is non-reactive with cells from SJL (H-2s) and NOD (H-2g7) mice.  Flow cytometric analysis indicates that the 2G9 and M5/114.15.2 monoclonal antibodies have comparable reactivity on cells from mice with I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek alloantigens.

      553621 Rev. 12
      Format Details
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      553621 Rev.12
      Citations & References
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      Development References (1)

      1. Becker D, Mohamadzadeh M, Reske K, Knop J. Increased level of intracellular MHC class II molecules in murine Langerhans cells following in vivo and in vitro administration of contact allergens. J Invest Dermatol. 1992; 99(5):545-549. (Immunogen: Immunofluorescence, Immunoprecipitation). View Reference
      553621 Rev. 12

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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