Skip to main content Skip to navigation
Hamburger Menu
Search icon
Order lookup icon Order lookup icon
Order Lookup
Country Selector Country Selector
United States (English)
Profile Icon Profile Icon
Sign-in/Register UserName
Products

Solutions

Discover & Learn

Resources & Tools

Support

Search icon Search icon
Close Menu
      Alert Icon
      FITC Rat Anti-Mouse Vβ4 T-Cell Receptor
      FITC Rat Anti-Mouse Vβ4 T-Cell Receptor
      Two-color analysis of the expression of Vβ4 TCR on peripheral T lymphocytes. C57BL/6 lymph node cells were incubated simultaneously with FITC-conjugated KT4, PE-conjugated RM4-5 (anti-CD4, Cat. no. 553048/553049), and PE-conjugated 53-6.7 (anti-CD8a, Cat. no. 553032/553033) monoclonal antibodies. Flow cytometry was performed on a FACScan™ (BDIS, San Jose, CA).
      FITC Rat Anti-Mouse Vβ4 T-Cell Receptor
      Two-color analysis of the expression of Vβ4 TCR on peripheral T lymphocytes. C57BL/6 lymph node cells were incubated simultaneously with FITC-conjugated KT4, PE-conjugated RM4-5 (anti-CD4, Cat. no. 553048/553049), and PE-conjugated 53-6.7 (anti-CD8a, Cat. no. 553032/553033) monoclonal antibodies. Flow cytometry was performed on a FACScan™ (BDIS, San Jose, CA).
      Product Details
      Down Arrow Up Arrow


      BD Pharmingen™
      Mouse (QC Testing)
      Rat SD, also known as Sprague-Dawley (outbred) IgG2b, κ
      KT4 B10.D2 mouse T-cell clone I3
      Flow cytometry (Routinely Tested)
      0.5 mg/ml
      AB_394811
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
      Show More blue down arrow Show Less blue up arrow

      Recommended Assay Procedures

      For flow cytometry of cell suspensions from peripheral lymphoid tissues, it is recommended that multicolor staining be performed to distinguish T lymphocytes from non-T cells.

      Show More blue down arrow Show Less blue up arrow

      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      Show More blue down arrow Show Less blue up arrow
      553365 Rev. 11
      Antibody Details
      Down Arrow Up Arrow
      KT4

      The KT4 antibody specifically recognizes the Vβ4 T-cell Receptor (TCR) of mice having the a (e.g., C57BR, C57L, SJL, SWR), b (e.g., A, AKR, BALB/c, CBA, C3H/He, C57BL, C58, DBA/1, DBA/2), and c (e.g., RIII) haplotypes of the Tcrb gene complex. Vβ4 TCR-bearing cells are among the CD4+ autoreactive T cells which induce autoimmune thyroiditis after elimination of regulatory (CD5-bright) T-cell subsets. Plate-bound KT4 antibody activates Vβ4 TCR-bearing T cells.

      553365 Rev. 11
      Format Details
      Down Arrow Up Arrow
      FITC
      Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      FITC
      Blue 488 nm
      494 nm
      518 nm
      553365 Rev.11
      Citations & References
      Down Arrow Up Arrow

      Development References (3)

      1. Katz JD, Benoist C, Mathis D. T helper cell subsets in insulin-dependent diabetes. Science. 1995; 268(5214):1185-1188. (Biology). View Reference
      2. Sugihara S, Fujiwara H, Shearer GM. Autoimmune thyroiditis induced in mice depleted of particular T cell subsets. Characterization of thyroiditis-inducing T cell lines and clones derived from thyroid lesions. J Immunol. 1993; 150(2):683-694. (Biology). View Reference
      3. Tomonari K, Lovering E, Spencer S. Correlation between the V beta 4+ CD8+ T-cell population and the H-2d haplotype. Immunogenetics. 1990; 31(5-6):333-339. (Immunogen). View Reference
      553365 Rev. 11

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

      Website Feedback