Purified NA/LE Rat Anti-Mouse CD49d

BD Pharmingen™ Purified NA/LE Rat Anti-Mouse CD49d

Name: Purified NA/LE Rat Anti-Mouse CD49d
Brand: BD Pharmingen™
SKU: 553313

Clone 9C10(MFR4.B)

(RUO)

Product Details

Brand: BD Pharmingen™

Alternative Name: Integrin α4; Itga4; Integrin alpha-4; LPAM alpha; VLA-4 alpha; VLA-4a

Reactivity: Mouse (QC Testing)

Isotype: Rat LEW, also known as Lewis IgG2a, κ

Immunogen: Mouse (C57BL/6xA/J)F[1] Fetal Liver Mast MC/9

Application: Flow cytometry (Routinely Tested), Blocking, Immunohistochemistry-frozen (Reported)

Concentration: 1.0 mg/ml

Entrez Gene ID: 16401

RRID: AB_394776

Storage Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

Companion Products

Purified NA/LE Rat IgG2a, κ Isotype Control RUO

Size 0.5 mg Cat No. 553926

FITC Goat Anti-Rat Ig RUO

Size 0.5 mg Cat No. 554016

Purified NA/LE Rat Anti-Mouse CD49d RUO

Size 0.5 mg Cat No. 553153

553313 Rev. 12

Antibody Details

9C10(MFR4.B)

The 9C10 (MFR4.B) monoclonal antibody specifically binds to the Integrin α4 chain (CD49d), which is expressed as a heterodimer with either of two β chains, β1 or β7. The α4β1 integrin (VLA-4, CD49d/CD29) is expressed on most peripheral lymphocytes, thymocytes, and monocytes; while the α4β7 integrin (LPAM-1) is expressed on peripheral lymphocytes, but on only a small subset of thymocytes. These integrins mediate a variety of cell-cell and cell-matrix interactions, recognizing the ligands VCAM-1 (CD106) and fibronectin. Integrin α4β7 also preferentially binds to the mucosal vascular addressin, MAdCAM-1. Although the 9C10 (MFR4.B) antibody alone has been reported to have little function-blocking activity, it can augment the inhibitory effects of mAb R1-2 (Cat. No. 553153), resulting in almost complete inhibition of VLA-4 binding to VCAM-1.

553313 Rev. 12

Format Details

NA/LE

NA/LE refers to the culture and purification methods and buffer used to produce purified antibodies with no azide and low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.NA/LE are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays — blocking, neutralizing, activation or depletion — where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.

Format: NA/LE

553313 Rev.12

Citations & References

Development References (2)

Halvorson MJ, Coligan JE. Enhancement of VLA integrin receptor function on thymocytes by cAMP is dependent on the maturation stage of the thymocytes. J Immunol. 1995; 155(10):4567-4574. (Clone-specific: Blocking). View Reference
Kinashi T, Springer TA. Adhesion molecules in hematopoietic cells. Blood Cells. 1994; 20(1):25-44. (Immunogen: Blocking). View Reference

553313 Rev. 12

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.