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      PE Rat Anti-Mouse Vα 11.1, 11.2[b,d] TCR
      PE Rat Anti-Mouse Vα 11.1, 11.2[b,d] TCR
      Two-color analysis of the expression of Vα 11.1, 11.2[b,d] TCR on peripheral lymphocytes. C57BL/6 lymph node cells were incubated simultaneously with PE-conjugated RR8-1, FITC-conjugated RM4-5 (anti-CD4, Cat. No. 553046/553047), and FITC-conjugated 53-6.7 (anti-CD8a, Cat. No. 553030/553031) monoclonal antibodies. Flow cytometry was performed on a FACScan™ (BDIS, San Jose, CA).
      PE Rat Anti-Mouse Vα 11.1, 11.2[b,d] TCR
      Two-color analysis of the expression of Vα 11.1, 11.2[b,d] TCR on peripheral lymphocytes. C57BL/6 lymph node cells were incubated simultaneously with PE-conjugated RR8-1, FITC-conjugated RM4-5 (anti-CD4, Cat. No. 553046/553047), and FITC-conjugated 53-6.7 (anti-CD8a, Cat. No. 553030/553031) monoclonal antibodies. Flow cytometry was performed on a FACScan™ (BDIS, San Jose, CA).
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      BD Pharmingen™
      Mouse (QC Testing)
      Rat IgG2b, κ
      Mouse T-Cell Clone B10
      Flow cytometry (Routinely Tested)
      0.2 mg/ml
      AB_394718
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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      Recommended Assay Procedures

      For flow cytometry of cell suspensions from peripheral lymphoid tissues, it is recommended that multicolor staining be performed to distinguish T lymphocytes from non-T cells. This antibody conjugate has been tested by immunofluorescent staining (≤ 1 μg/million cells) with flow cytometric analysis.

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      553223 Rev. 2
      Antibody Details
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      RR8-1

      The RR8-1 monoclonal antibody specifically recognizes the Vα 11.1 and Vα 11.2, but not Vα 11.3 T-cell Receptors (TCR) of mice having the b (e.g., C57BL) and d (e.g., DBA/1, DBA/2, NZW) haplotypes of Tcra gene complex. RR8-1 antibody does not react with strains having the a (e.g., A, AKR, BALB/c, CBA, C3H/He) or c (e.g., NZB, SJL, SWR, NOD) Tcra haplotypes. Plate-bound RR8-1 antibody activates Vα 11.1, 11.2[b,d] TCR-bearing T lymphocytes.

      553223 Rev. 2
      Format Details
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      PE
      R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      PE
      Yellow-Green 488 nm, 532 nm, 561 nm
      496 nm, 566 nm
      576 nm
      553223 Rev.2
      Citations & References
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      Development References (1)

      1. Jameson SC, Nakajima PB, Brooks JL, Heath W, Kanagawa O, Gascoigne NR. The T cell receptor V alpha 11 gene family. Analysis of allelic sequence polymorphism and demonstration of J alpha region-dependent recognition by allele-specific antibodies. J Immunol. 1991; 147(9):3185-3193. (Immunogen). View Reference
      553223 Rev. 2

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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