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      Purified Rat Anti-Mouse CD200
      Purified Rat Anti-Mouse CD200
      Multi-color analysis of the expression of CD200 on thymocytes. C57BL/6 thymocytes were stained with either purified mAb OX-90 (open histograms) or purified rat IgG2a, κ isotype control mAb R35-95 (Cat. No. 553927, shaded histograms), followed by biotinylated anti-rat IgG2a mAb RG7/1.30 (Cat. No. 553894) then Streptavidin-PE (Cat. No. 554061). Then the cells were stained with FITC-conjugated anti-mouse CD4 mAb and APC-conjugated anti-mouse CD8a mAb 53-6.7 (Cat. No. 553035). Electronic gates were set to include the CD4-CD8a-, CD4+CD8a+, CD4+CD8a-, and CD4-CD8a+ populations, and their expression of CD200 is displayed in the corresponding panels. Flow cytometry was performed on a BD FACSCalibur™ Flow Cytometry System.
      Purified Rat Anti-Mouse CD200
      Multi-color analysis of the expression of CD200 on thymocytes. C57BL/6 thymocytes were stained with either purified mAb OX-90 (open histograms) or purified rat IgG2a, κ isotype control mAb R35-95 (Cat. No. 553927, shaded histograms), followed by biotinylated anti-rat IgG2a mAb RG7/1.30 (Cat. No. 553894) then Streptavidin-PE (Cat. No. 554061). Then the cells were stained with FITC-conjugated anti-mouse CD4 mAb and APC-conjugated anti-mouse CD8a mAb 53-6.7 (Cat. No. 553035). Electronic gates were set to include the CD4-CD8a-, CD4+CD8a+, CD4+CD8a-, and CD4-CD8a+ populations, and their expression of CD200 is displayed in the corresponding panels. Flow cytometry was performed on a BD FACSCalibur™ Flow Cytometry System.
      Product Details
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      BD Pharmingen™
      Cd200; OX2; OX-2; OX2G; MRC OX-2 antigen; Mox2
      Mouse (QC Testing)
      Rat IgG2a, κ
      Soluble fusion protein of the extracellular region of mouse OX-2 antigen w/domains 3 & 4 of rat CD4
      Flow cytometry (Routinely Tested), ELISA, Immunohistochemistry (Reported)
      0.5 mg/ml
      17470
      AB_394410
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
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      Recommended Assay Procedures

      Since this antigen is expressed at low density on resting peripheral leukocytes, it may be desirable to amplify staining by using a biotinylated second-step antibody followed by a "bright" third-step reagent, such as Streptavidin-PE (Cat. No. 554061). We also recommend the use of Mouse BD Fc Block™ (anti-mouse CD16/CD32, mAb 2.4G2, Cat. No. 553141/553142) when Fc Receptor-bearing cells are present. For staining with purified antibody in the presence of Mouse BD Fc Block™, a second-step antibody which does not react with the 2.4G2 antibody (Rat IgG2b, κ) must be used. We have found that biotinylated anti-rat IgG2a mAb RG7/1.30 (Cat. No. 553894) is useful.

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
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      552512 Rev. 1
      Antibody Details
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      OX-90

      The OX-90 monoclonal antibody specifically recognizes CD200 (OX-2 antigen), a type-1 transmembrane glycoprotein containing two extracellular Ig-like domains. CD200 is expressed on thymocytes, neurons, B lymphocytes, splenic follicular dendritic cells and endothelium, and subsets of T lymphocytes and dendritic cells; but not on NK cells, granulocytes, monocytes, or macrophages. CD200 Receptor (CD200R; OX2R) expression is restricted to myeloid cells, and it appears that its engagement with CD200 results in inhibition and/or down-regulation of myeloid cell activity.

      552512 Rev. 1
      Format Details
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      552512 Rev.1
      Citations & References
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      Development References (4)

      1. Barclay NA, Brown MH, Birkeland ML, et al, ed. The Leukocyte Antigen FactsBook. San Diego, CA: Academic Press; 1997.
      2. Gorczynski L, Chen Z, Hu J, et al. Evidence that an OX-2-positive cell can inhibit the stimulation of type 1 cytokine production by bone marrow-derived B7-1 (and B7-2)-positive dendritic cells. J Immunol. 1999; 162(2):774-781. (Biology). View Reference
      3. Hoek RM, Ruuls SR, Murphy CA, et al. Down-regulation of the macrophage lineage through interaction with OX2 (CD200).. Science. 2000; 290(5497):1768-71. (Immunogen: ELISA, Immunohistochemistry). View Reference
      4. Preston S, Wright GJ, Starr K, Barclay AN, Brown MH. The leukocyte/neuron cell surface antigen OX2 binds to a ligand on macrophages. Eur J Immunol. 1997; 27(8):1911-1918. (Immunogen). View Reference
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      552512 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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