Skip to main content Skip to navigation
PE Mouse Anti-Human CD124
PE Mouse Anti-Human CD124
Flow cytometric analysis of CD124 expression on human PBMC. Human PBMC isolated by density gradient centrifugation (Ficoll-Paque™) were blocked with either normal polyclonal human IgG and stained with PE Mouse Anti-Human CD124 (Cat. No. 552178; filled histogram) or PE Mouse IgG1, κ Isotype Control (Cat. No. 555749; open histogram) at at 20 µl/10^6 cells. Fluorescence histograms depicting CD124 (or Ig isotype) expression were derived from gated events with the forward and side light-scatter characteristics of viable CD19+ lymphocytes.   Note: Certain human cell lines or cell types (e.g., neutrophils and monocytes) can first be treated with reagents that block receptors for the Fc regions of immunoglobulin to avoid nonspecific immunofluorescent staining mediated by Fc receptors.
Flow cytometric analysis of CD124 expression on human PBMC. Human PBMC isolated by density gradient centrifugation (Ficoll-Paque™) were blocked with either normal polyclonal human IgG and stained with PE Mouse Anti-Human CD124 (Cat. No. 552178; filled histogram) or PE Mouse IgG1, κ Isotype Control (Cat. No. 555749; open histogram) at at 20 µl/10^6 cells. Fluorescence histograms depicting CD124 (or Ig isotype) expression were derived from gated events with the forward and side light-scatter characteristics of viable CD19+ lymphocytes.   Note: Certain human cell lines or cell types (e.g., neutrophils and monocytes) can first be treated with reagents that block receptors for the Fc regions of immunoglobulin to avoid nonspecific immunofluorescent staining mediated by Fc receptors.
Product Details
Down Arrow Up Arrow


BD Pharmingen™
IL-4 Receptor α Chain, CD124
Human (QC Testing)
Mouse IgG1, κ
Soluble Human IL-4 Receptor
Flow cytometry (Routinely Tested)
20 µl
V C004, BP169; VI BP205, C81
3566
AB_394355
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Recommended Assay Procedures

Immunofluorescent Staining and Flow Cytometric Analysis: The PE Mouse Anti-Human CD124 (Cat. No. 554178) antibody can be used for the immunofluorescent staining (20 µl/10^6 cells) and flow cytometric analysis of human nucleated cells to measure their expressed levels of surface hIL-4Rα. An appropriate purified immunoglobulin isotype control is PE Mouse IgG1, κ Isotype Control (Cat. No. 555749).

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. Ficoll-Paque is a trademark of Amersham Biosciences Limited.
  7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
552178 Rev. 2
Antibody Details
Down Arrow Up Arrow
hIL4R-M57

The hIL4R-M57 antibody specifically binds to the α subunit (IL-4Rα) of the human Interleukin-4 Receptor complex which is also known as CD124. The human IL-4Rα, also known as B cell stimulatory factor 1 receptor (BSF-1 receptor), is a 140 kDa transmembrane glycoprotein that is expressed by B and T lymphocytes and a variety of other hematopoietic and non-hematopoietic cells and cell lines. The cell surface IL-4Rα chain binds IL-4 with high affinity and associates with either the common γ chain (IL-4Rα/γc; aka, type I IL-4R complex) or the IL-13 receptor alpha-1 subunit (IL-4Rα/IL-13Rα1; aka, type II IL-4R complex) to form two distinct types of signal-transducing IL-4R complexes. The type I IL-4 receptor complex specifically binds IL-4 whereas the type II IL-4R complex binds and transduces signals from either IL-4 or IL-13. A truncated form of the IL-4Rα exists in soluble form in biological fluids. In contrast to mice, in humans no distinct mRNA coding for sIL-4R has been described, suggested that human sIL4-R is exclusively produced by proteolytic cleavage of the cell surface receptor. The serum levels of soluble IL-4Rα appear to elevate in pathological situations such as allergy and parasitic infections. Depending on the ratios of IL-4 and sIL-4Rα present in the local milieu, the sIL-4Rα may augment or antagonize the activities of IL-4. The immunogen used to generate the hIL4R-M57 hybridoma was soluble human IL-4R.

552178 Rev. 2
Format Details
Down Arrow Up Arrow
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
552178 Rev.2
Citations & References
Down Arrow Up Arrow

Development References (19)

  1. Armitage RJ, Beckmann MP, Idzerda RL, Alpert A, Fanslow WC. Regulation of interleukin 4 receptors on human T cells. Int Immunol. 1990; 2(11):1039-1045. (Biology). View Reference
  2. Armitage RJ, Ziegler SF, Beckmann MP, Idzerda RL, Park LS, Fanslow WC. Expression of receptors for interleukin 4 and interleukin 7 on human T cells. Adv Exp Med Biol. 1991; 292:121-130. (Biology). View Reference
  3. Browning JL, Dougas I, Ngam-ek A, et al. Characterization of surface lymphotoxin forms. Use of specific monoclonal antibodies and soluble receptors.. J Immunol. 1995; 154(1):33-46. (Biology). View Reference
  4. Fanslow WC, Spriggs MK, Rauch CT, et al. Identification of a distinct low-affinity receptor for human interleukin-4 on pre-B cells. Blood. 1993; 81(11):2998-3005. (Biology). View Reference
  5. Gessner A, Rollinghoff M. Biologic functions and signaling of the interleukin-4 receptor complexes. Immunobiology. 2000; 201(3-4):285-307. (Biology). View Reference
  6. Hoffman RC, Castner BJ, Gerhart M, et al. Direct evidence of a heterotrimeric complex of human interleukin-4 with its receptors. Protein Sci. 1995; 4(3):382-386. (Biology). View Reference
  7. Idzerda RL, March CJ, Mosley B, et al. Human interleukin 4 receptor confers biological responsiveness and defines a novel receptor superfamily. J Exp Med. 1990; 171(3):861-873. (Clone-specific). View Reference
  8. Jung T, Bews JP, Enssle KH, Wagner K, Neumann C, Heusser CH. Detection of and discrimination between total and free human interleukin-4 and free soluble interleukin-4 receptor by ELISA. J Immunol Methods. 1998; 217(1-2):41-50. (Biology). View Reference
  9. Jung T, Schrader N, Hellwig M, Enssle KH, Neumann C. Soluble human interleukin-4 receptor is produced by activated T cells under the control of metalloproteinases. Int Arch Allergy Immunol. 1999; 119(1):23-30. (Biology). View Reference
  10. Law CL, Armitage RJ, Villablanca JG, LeBien TW. Expression of interleukin-4 receptors on early human B-lineage cells. Blood. 1991; 78(3):703-710. (Biology). View Reference
  11. Mozo L, Rivas D, Zamorano J, Gutierrez C. Differential expression of IL-4 receptors in human T and B lymphocytes. J Immunol. 1993; 150(10):4261-4269. (Biology). View Reference
  12. Nasert S, Millner M, Enssle KH, Wahn U, Renz H. Differential modulation of T cell functions by soluble IL-4R (s1L-4R) in two cases of severe atopic dermatitis. Pediatr Allergy Immunol. 1996; 7(2):91-94. (Biology). View Reference
  13. Ohara J, Paul WE. Receptors for B-cell stimulatory factor-1 expressed on cells of haematopoietic lineage. Nature. 1987; 325(6104):537-540. (Biology). View Reference
  14. Park LS, Friend D, Sassenfeld HM, Urdal DL. Characterization of the human B cell stimulatory factor 1 receptor. J Exp Med. 1987; 166(2):476-488. (Biology). View Reference
  15. Sang DK, Ouma JH, John CC, et al. Increased levels of soluble interleukin-4 receptor in the sera of patients with visceral leishmaniasis. J Infect Dis. 1999; 179(3):743-746. (Biology). View Reference
  16. Zola H, Flego L, Weedon H. Expression of IL-4 receptor on human T and B lymphocytes. Cell Immunol. 1993; 150(1):149-158. (Clone-specific: Flow cytometry). View Reference
  17. Zuber CE, Galizzi JP, Harada N, Durand I, Banchereau J. Interleukin-4 receptors on human blood mononuclear cells. Cell Immunol. 1990; 129(2):329-340. (Biology). View Reference
  18. Zuber CE, Galizzi JP, Valle A, Harada N, Howard M, Banchereau J. Interleukin 4 receptors on normal human B lymphocytes: characterization and regulation. Eur J Immunol. 1990; 20(3):551-555. (Biology). View Reference
  19. Zurawski SM, Chomarat P, Djossou O, et al. The primary binding subunit of the human interleukin-4 receptor is also a component of the interleukin-13 receptor. J Biol Chem. 1995; 270(23):13869-13878. (Biology). View Reference
View All (19) View Less
552178 Rev. 2

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.