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      PE Mouse Anti-Rat γδ T-Cell Receptor
      PE Mouse Anti-Rat γδ T-Cell Receptor
      Two-color analysis of the expression of γδ TCR on peripheral lymphocytes. Lewis lymph node cells were incubated simultaneously with PE-conjugated mAb V65 (right panel) and FITC-conjugated anti-rat CD3 mAb G4.18 (Cat. No. 559975/554832). Flow cytometry was performed on a BD FACScan™ flow cytometry system.
      PE Mouse Anti-Rat γδ T-Cell Receptor
      Two-color analysis of the expression of γδ TCR on peripheral lymphocytes. Lewis lymph node cells were incubated simultaneously with PE-conjugated mAb V65 (right panel) and FITC-conjugated anti-rat CD3 mAb G4.18 (Cat. No. 559975/554832). Flow cytometry was performed on a BD FACScan™ flow cytometry system.
      Product Details
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      BD Pharmingen™
      Rat (QC Testing)
      Mouse BALB/c IgG1, κ
      Rat CD3+ αβ TCR- T-cell hybridoma III.89.1.4
      Flow cytometry (Routinely Tested)
      0.2 mg/ml
      AB_394257
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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      Recommended Assay Procedures

      For flow cytometry of cell suspensions from peripheral lymphoid tissues, it is recommended that multicolor staining be performed on to distinguish T lymphocytes from non-T cells.

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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      551802 Rev. 10
      Antibody Details
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      V65

      The V65 monoclonal antibody specifically recognizes the γδ T-cell Receptor (TCR) found on CD3+ αβ  TCR-peripheral T lymphocytes, on a small subset of intestinal intraepithelial lymphocytes, and on dendritic epidermal T cells.  Cross-linked V65 mAb induces cytokine-dependent T-cell mitogenesis in vitro, and in vivo treatment can significantly deplete peripheral γδ TCR-expressing T cells. A suspension of rat CD3+ αβ  TCR-T-cell hybridoma III.89.1.4 was used as the source of the immunogen.

      551802 Rev. 10
      Format Details
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      PE
      R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      PE
      Yellow-Green 488 nm, 532 nm, 561 nm
      496 nm, 566 nm
      576 nm
      551802 Rev.10
      Citations & References
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      Development References (2)

      1. Kuhnlein P, Park JH, Herrmann T, Elbe A, Hunig T. Identification and characterization of rat gamma/delta T lymphocytes in peripheral lymphoid organs, small intestine, and skin with a monoclonal antibody to a constant determinant of the gamma/delta T cell receptor. J Immunol. 1994; 153(3):979-986. (Immunogen). View Reference
      2. Pelegri C, Kuhnlein P, Buchner E, et al. Depletion of gamma/delta T cells does not prevent or ameliorate, but rather aggravates, rat adjuvant arthritis. Arthritis Rheum. 1996; 39(2):204-215. (Biology). View Reference
      551802 Rev. 10

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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