FITC Rat Anti-Rat CD45.1

BD Pharmingen™ FITC Rat Anti-Rat CD45.1

Name: FITC Rat Anti-Rat CD45.1
Brand: BD Pharmingen™
SKU: 550802

Clone NDS58

(RUO)

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Product Details

Brand: BD Pharmingen™

Alternative Name: RT7.1 of LCA

Reactivity: Rat (QC Testing)

Isotype: Rat AS IgG2b, κ

Immunogen: LEW rat thymocytes

Application: Flow cytometry (Routinely Tested)

Concentration: 0.5 mg/ml

RRID: AB_393888

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Companion Products

FITC Rat IgG2b, κ Isotype Control RUO

Size 0.25 mg Cat No. 553988

550802 Rev. 3

Antibody Details

NDS58

The NDS58 antibody reacts with all isoforms of CD45 (Leukocyte Common Antigen) on all hematopoietic cells, excluding the more mature erythroid cells, in strains expressing the RT7.1 alloantigen. The antibody does not react with the RT7.2 alloantigen. RT7.1 strains include ACI, AO, BN, DA, DZB, LEW, MAXX, PVG, SHR, and WAG; while BUF, LOU, and WF are RT7.2 strains. CD45 is a member of the Protein Tyrosine Phosphatase (PTP) family: Its intracellular (COOH-terminal) region contains two PTP catalytic domains, and the extracellular region is highly variable due to alternative splicing of exons 4, 5, and 6 (designated A, B, and C, respectively), plus differing levels of glycosylation. The CD45 isoforms detected in the rat are cell type-, maturation-, and activation state-specific. The CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction.

550802 Rev. 3

Format Details

FITC

Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: FITC

Excitation Source: Blue 488 nm

Excitation Max: 494 nm

Emission Max: 518 nm

550802 Rev.3

Citations & References

Development References (4)

Butcher GW. A list of monoclonal antibodies specific for alloantigens of the rat. J Immunogenet. 1987; 14(2-3):163-176. (Biology). View Reference
Johnson P, Maiti A, Ng DHW. CD45: A family of leukocyte-specific cell surface glycoproteins. In: Herzenberg LA, Weir DM, Herzenberg LA, Blackwell C , ed. Weir's Handbook of Experimental Immunology, Vol 2. Cambridge: Blackwell Science; 1997:62.1-62.16.
Kampinga J, Kroese FG, Pol GH, et al. RT7-defined alloantigens in rats are part of the leucocyte common antigen family. Scand J Immunol. 1990; 31(6):699-710. (Biology). View Reference
Newton MR, Wood KJ, Fabre JW. A monoclonal alloantibody detecting a polymorphism of the rat leucocyte common (LC) antigen. J Immunogenet. 1986; 13(1):41-50. (Immunogen). View Reference

View All (4)

550802 Rev. 3

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.