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      CD45 PerCP
      Product Details
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      BD™
      PTPRC; LCA; L-CA; Leukocyte Common Antigen; T200; GP180; LY5
      Human
      Mouse IgG1, κ
      Flow cytometry
      25 μg/mL
      20 μL
      Phosphate buffered saline with gelatin and sodium azide.
      ASR


      Preparation And Storage

      Store vials at 2°C to 8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

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      340665 Rev. 1
      Antibody Details
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      2D1

      The CD45 antibody, clone 2D1, is derived from the hybridization of NS-1 mouse myeloma cells with spleen cells from BALB/c mice immunized with human peripheral blood mononuclear cells (PBMCs).

      The CD45 antibody (anti–HLe-1) recognizes members of the T200 family of human leucocyte antigens with molecular mass of 180 to 220 kilodaltons (kDa.)

      340665 Rev. 1
      Format Details
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      PerCP
      PerCP dye is part of the BD blue family of dyes. This dye is a fluorescent protein complex with an excitation maximum (Ex Max) of 481 nm and an emission maximum (Em Max) at 675 nm. PerCP is designed to be excited by the blue laser (488 nm) and detected using an optical filter centered near 680 nm (e.g., a 695/40 nm bandpass filter). The donor dye can be partially excited by the Violet (405 nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      PerCP
      Blue 488 nm
      481 nm
      675 nm
      340665 Rev.1
      Citations & References
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      Development References (4)

      1. Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
      2. Clinical and Laboratory Standards Institute. 2005. (Biology).
      3. Jackson A. Basic phenotyping of lymphocytes: selection and testing of reagents and interpretation of data. Clin Immunol Newslett. 1990; 10:43-55. (Biology).
      4. Schwinzer R. Cluster Report: CD45/CD45R. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:628-634.
      View All (4) View Less
      340665 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      Analyte Specific Reagent. Analytical and performance characteristics are not established.

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