Alexa Fluor® 647 Mouse anti-Human IL-21
Clone 3A3-N2.1 (also known as 3A3-N2) (RUO)
- Brand BD Pharmingen™
- Alternative Name Interleukin-21, IL21, Za11
- Vol. Per Test 20 µl
- Isotype Mouse IgG1, κ
- Reactivity Human (QC Testing)
Intracellular staining (flow cytometry) (Routinely Tested)
- Immunogen Human IL-21 Recombinant Protein
- Storage Buffer Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
Human Interleukin-21 (IL-21) is a member of the type I cytokine family that is encoded by a gene resident on chromosome 4. The mature form of human IL-21 is a 131 amino acid protein. IL-21 is produced by activated NKT and multiple CD4+ T cell subsets including effector memory and central memory CD4+ T cells and differentiated T helper cell subsets polarized towards Th17 cell and T follicular helper (Tfh) phenotypes. IL-21 plays important protective roles in the regulation of hematopoiesis and both innate and adaptive immune responses and adverse roles in promoting autoimmunity. IL-21 costimulates the proliferation and differentiation of CD4+ T cells. It enhances the proliferation of and cytotoxicity mediated by natural killer (NK) cells and CD8+ T cells. IL-21 costimulates B cell proliferation and differentiation into plasma cells producing immunoglobulins with IgG isotypes. IL-21 can also regulate the functions of dendritic cells and other myeloid cells. IL-21 exerts its biological activities by binding to and activating the Janus activating kinases (JAK1 and JAK3) and signal transducers and activators of transcription (STAT1, STAT3, STA5a and STAT5b) signaling pathways through the IL-21 receptor (IL-21R) complex. The IL-21R complex is comprised of the IL-21R alpha subunit and the common cytokine receptor gamma subunit (γ c; CD132).
The monoclonal 3A3-N2 antibody specifically binds to human IL-21.
Alexa Fluor® 647 conjugates are highly photostable and remain fluorescent over a broad pH range. The excitation and emission maxima are nearly identical to those of APC. However, APC tends to be brighter while Alexa Fluor® 647 is more optimal for intracellular applications. This fluorochrome exhibits uncommon photostability, making it an ideal choice for use in fluorescence microscopy. Due to nearly identical excitation and emission properties but different spillover characteristics, APC and Alexa Fluor® 647 cannot be used simultaneously.
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Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- An isotype control should be used at the same concentration as the antibody of interest.