Left Panel - CXCL1 (GROα) expression in human peripheral blood mononuclear cells. PBMCs were cultured without (Left Plots) or with (Right Plots) lipopolysaccharide (LPS) (100 ng/ml, 6 h, 37°C) and BD GolgiStop™ Protein Transport Inhibitor (containing Monensin) (Cat. No. 554724). Cells were harvested, washed, fixed and permeabilized with BD Cytofix/Cytoperm™ Fixation and Permeabilization Solution (Cat. No. 554722). Cells were then washed and stained in BD Perm/Wash™ Buffer (Cat. No. 554723) with BD Horizon™ BV421 Mouse Anti-Human CD14 (Cat. No. 565283) and either Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (Cat. No. 566011; Top Plots) or Alexa Fluor® 647 Mouse Anti-Human CXCL1 (GROα) antibody (Cat. No. 566579; Bottom Plots) at 0.5 ug/test. Two-color flow cytometric contour plots showing the correlated expression of CXCL1 (or Ig Isotype control staining) versus CD14 were derived from gated events with the forward and side light-scatter characteristics of intact cells. Right Panel - CXCL1 (GROα) expression in HiCK-3 Human Cytokine Positive Control Cells. HiCK-3 cells (Cat. No. 555063) were washed and permeabilized using BD Perm/Wash™ Buffer. The cells were stained with BD Horizon BV421 Mouse Anti-Human CD14 and either Alexa Fluor® 647 Mouse IgG1, κ Isotype Control Top Plot) or Alexa Fluor® 647 Mouse Anti-Human CXCL1 (GROα) antibody (Bottom Plot) at 0.5 μg/test. Two-color flow cytometric contour plots showing the correlated expression of CXCL1 (or Ig Isotype control staining) versus CD14 were derived from gated events with the forward and side light-scatter characteristics of intact PBMCs. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific.
Left Panel - CXCL1 (GROα) expression in human peripheral blood mononuclear cells. PBMCs were cultured without (Left Plots) or with (Right Plots) lipopolysaccharide (LPS) (100 ng/ml, 6 h, 37°C) and BD GolgiStop™ Protein Transport Inhibitor (containing Monensin) (Cat. No. 554724). Cells were harvested, washed, fixed and permeabilized with BD Cytofix/Cytoperm™ Fixation and Permeabilization Solution (Cat. No. 554722). Cells were then washed and stained in BD Perm/Wash™ Buffer (Cat. No. 554723) with BD Horizon™ BV421 Mouse Anti-Human CD14 (Cat. No. 565283) and either Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (Cat. No. 566011; Top Plots) or Alexa Fluor® 647 Mouse Anti-Human CXCL1 (GROα) antibody (Cat. No. 566579; Bottom Plots) at 0.5 ug/test. Two-color flow cytometric contour plots showing the correlated expression of CXCL1 (or Ig Isotype control staining) versus CD14 were derived from gated events with the forward and side light-scatter characteristics of intact cells. Right Panel - CXCL1 (GROα) expression in HiCK-3 Human Cytokine Positive Control Cells. HiCK-3 cells (Cat. No. 555063) were washed and permeabilized using BD Perm/Wash™ Buffer. The cells were stained with BD Horizon BV421 Mouse Anti-Human CD14 and either Alexa Fluor® 647 Mouse IgG1, κ Isotype Control Top Plot) or Alexa Fluor® 647 Mouse Anti-Human CXCL1 (GROα) antibody (Bottom Plot) at 0.5 μg/test. Two-color flow cytometric contour plots showing the correlated expression of CXCL1 (or Ig Isotype control staining) versus CD14 were derived from gated events with the forward and side light-scatter characteristics of intact PBMCs. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific.
Product Details
BD Pharmingen™
C-X-C motif chemokine 1; FSP; GRO; GROA; GRO1; MGSA; NAP-3; SCYB1
Human (QC Testing)
Mouse BALB/c IgG1, κ
Human CXCL1 Recombinant Protein
Intracellular staining (flow cytometry) (Routinely Tested)
0.2 mg/ml
AB_2739746
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 mL
Cat No.
554657
Fixation and Permeabilization Solution RUO
Size
125 mL
Cat No.
554722
Perm/Wash Buffer RUO
Size
100 mL
Cat No.
554723
Protein Transport Inhibitor (Containing Monensin) RUO
Size
0.7 mL
Cat No.
554724
Alexa Fluor® 647 Mouse IgG1, κ Isotype Control RUO
Size
50 µg
Cat No.
566011
566579 Rev. 1
Antibody Details
V40-275
The V40-275 monoclonal antibody specifically recognizes C-X-C motif chemokine ligand 1 (CXCL1) which is also known as Growth-regulated alpha protein (GRO alpha or GROα), Melanoma growth stimulatory activity (MGSA), Fibroblast secretory protein (FSP), Neutrophil-activating protein 3 (NAP-3), or SCYB1. CXCL1 (GROα) is a proinflammatory chemokine that is produced by activated monocytes, macrophages, Kupffer cells, mast cells, fibroblasts, epithelial and endothelial cells in response to proinflammatory stimuli including lipopolysaccharide (LPS), Interleukin-1 (IL-1), or TNF. CXCL1 (GROα) binds to CD182, the CXCR2 chemokine receptor, to attract and activate neutrophils, and to promote angiogenesis and fibrogenesis. Overexpression of CXCL1 (GROα) in some malignant tumors may contribute to tumor vascularization and metastasis.
566579 Rev. 1
Format Details
Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
566579 Rev.1
Citations & References
Development References (6)
-
Anisowicz A, Bardwell L, Sager R. Constitutive overexpression of a growth-regulated gene in transformed Chinese hamster and human cells. Proc Natl Acad Sci U S A. 1987; 84(20):7188-7192. (Biology). View Reference
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Haskill S, Beg AA, Tompkins SM, et al. Characterization of an immediate-early gene induced in adherent monocytes that encodes I kappa B-like activity. Cell. 1991; 65(7):1281-1289. (Biology). View Reference
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Iida N, Grotendorst GR. Cloning and sequencing of a new gro transcript from activated human monocytes: expression in leukocytes and wound tissue. Mol Cell Biol. 1990; 10(10):5596-5599. (Biology). View Reference
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Nischalke HD, Berger C, Luda C, et al. The CXCL1 rs4074 A allele is associated with enhanced CXCL1 responses to TLR2 ligands and predisposes to cirrhosis in HCV genotype 1-infected Caucasian patients.. J Hepatol. 2012; 56(4):758-64. (Biology). View Reference
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Richmond A, Balentien E, Thomas HG. Molecular characterization and chromosomal mapping of melanoma growth stimulatory activity, a growth factor structurally related to beta-thromboglobulin. EMBO J. 1988; 7(7):2025-2033. (Biology). View Reference
-
Yang J, Fan GH, Wadzinski BE, Sakurai H, Richmond A. Protein phosphatase 2A interacts with and directly dephosphorylates RelA. J Biol Chem. 2002; 276(51):47828-47833. (Biology). View Reference
566579 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
