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      PE Mouse Anti-Human IL-9
      PE Mouse Anti-Human IL-9
      Flow cytometric analysis of IL-9 in stimulated human CD4-positive T cells. Human peripheral blood mononuclear cells were stimulated in a tissue culture plate coated with NA/LE Mouse Anti-Human CD3 (Cat. No. 555329; 10 µg/ml, coated overnight at 4°C) and soluble NA/LE Mouse Anti-Human CD28 (Cat. No. 555725; 1 µg/ml) antibodies plus recombinant Human IL-2 (Cat. No. 554603; 10 ng/ml), IL-4 (Cat. No. 554605; 50 ng/ml), and TGF-β (Cat. No. 356039; 10 ng/ml) proteins and NA/LE Mouse Anti-Human IFN-γ (Cat. No. 554698; 10 µg/ml) antibody for 5 days. The cells were harvested and restimulated with PMA (Sigma P8139; 50 ng/ml) and ionomycin (Sigma I9657; 1 µg/ml) in the presence of BD GolgiStop™ Protein Transport Inhibitor (Cat. No. 554724) for 5 hours. The cells were then fixed and permeabilized using the BD Cytofix/Cytoperm™ Fixation/Permeablization Kit (Cat. No. 554714) followed by staining with PE Mouse Anti-Human IL-9 (Cat. No. 560807) and APC Mouse Anti-Human CD4 (Cat. No. 555349) antibodies. Two-color flow cytometric dot plots showing correlated expression patterns of CD4 versus IL-9 were derived from CD4-positive cell gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed on a BD LSRII™ System.
      PE Mouse Anti-Human IL-9
      Flow cytometric analysis of IL-9 in stimulated human CD4-positive T cells. Human peripheral blood mononuclear cells were stimulated in a tissue culture plate coated with NA/LE Mouse Anti-Human CD3 (Cat. No. 555329; 10 µg/ml, coated overnight at 4°C) and soluble NA/LE Mouse Anti-Human CD28 (Cat. No. 555725; 1 µg/ml) antibodies plus recombinant Human IL-2 (Cat. No. 554603; 10 ng/ml), IL-4 (Cat. No. 554605; 50 ng/ml), and TGF-β (Cat. No. 356039; 10 ng/ml) proteins and NA/LE Mouse Anti-Human IFN-γ (Cat. No. 554698; 10 µg/ml) antibody for 5 days. The cells were harvested and restimulated with PMA (Sigma P8139; 50 ng/ml) and ionomycin (Sigma I9657; 1 µg/ml) in the presence of BD GolgiStop™ Protein Transport Inhibitor (Cat. No. 554724) for 5 hours. The cells were then fixed and permeabilized using the BD Cytofix/Cytoperm™ Fixation/Permeablization Kit (Cat. No. 554714) followed by staining with PE Mouse Anti-Human IL-9 (Cat. No. 560807) and APC Mouse Anti-Human CD4 (Cat. No. 555349) antibodies. Two-color flow cytometric dot plots showing correlated expression patterns of CD4 versus IL-9 were derived from CD4-positive cell gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed on a BD LSRII™ System.
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      BD Pharmingen™
      IL9; IL-9; interleukin-9; HP40; P40
      Human (QC Testing)
      Mouse C57BL/6 IgG1, κ
      Human IL-9 Recombinant Protein
      Intracellular staining (flow cytometry) (Routinely Tested)
      5 µl
      AB_2033985
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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      Product Notices

      1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
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      Antibody Details
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      MH9A3

      The MH9A3 monoclonal antibody specifically binds to human interleukin-9 (IL-9). Human IL-9 is a multifunctional cytokine and a member of the type I cytokine (hematopoietin) family that includes IL-2, IL-4, IL-7, IL-15 and IL-21. This cytokine is encoded by the IL9 gene that is resident on chromosome 5q31.1. IL-9 is expressed by activated CD4-positive T helper cells, by some transformed T cells and by eosinophils, mast cells and neutrophils. IL-9 induces the proliferation, differentiation, and effector function of various cell types including T lymphocytes, B lymphocytes, mast cells, eosinophils, neutrophils, hematopoietic cells and epithelial cells. It potentiates the interleukin-4-induced IgM, IgG and IgE responses by human B lymphocytes. IL-9 has been implicated in human allergic disorders such as asthma and malignancies such as Hodgkin's disease. IL-9 exerts its biological activities through binding to the surface IL-9 receptor (IL-9R) complex comprised of the IL-9R alpha subunit (IL-9Rα; CD129) and the common cytokine receptor gamma subunit (γc; CD132). IL-9 signaling through its receptor includes activation of the Janus kinases 1 and 3 ( JAK1 and JAK3) and activation of Signal transducer and activator of transcription 1, 3 and 5 factors (STAT1, STAT3 and STAT5).

      Format Details
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      PE
      R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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      PE
      Yellow-Green 488 nm, 532 nm, 561 nm
      496 nm, 566 nm
      576 nm
      Citations & References
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      Development References (9)

      1. Demoulin JB, Van Roost E, Stevens M, Groner B, Renauld JC. Distinct roles for STAT1, STAT3, and STAT5 in differentiation gene induction and apoptosis inhibition by interleukin-9. J Biol Chem. 1999; 274:25855-25861. (Biology). View Reference
      2. Dugas B, Renauld JC, Pène J, et al. Interleukin-9 potentiates the interleukin-4-induced immunoglobulin (IgG, IgM and IgE) production by normal human B lymphocytes. Eur J Immunol. 1993 July; 23(7):1687-1692. (Biology). View Reference
      3. Houssiau FA, Schandene L, Stevens M. A cascade of cytokines is responsible for IL-9 expression in human T cells. Involvement of IL-2, IL-4, and IL-10. J Immunol. 1995; 154(6):2624-2630. (Biology). View Reference
      4. Jenmalm MC, Van Snick J, Cormont F, Salman B. Allergen-induced Th1 and Th2 cytokine secretion in relation to specific allergen sensitization and atopic symptoms in children. Clin Exp Allergy. 2001 October; 31(10):1528-1535. (Clone-specific: ELISA). View Reference
      5. Knoops L, Renauld JC. IL-9 and its receptor: from signal transduction to tumorigenesis. Growth Factors. 2004; 22:207-215. (Biology). View Reference
      6. Merz H, Houssiau FA, Orscheschek K, et al. Interleukin-9 expression in human malignant lymphomas: unique association with Hodgkin's disease and large cell anaplastic lymphoma. Blood. 1991 September; 78(5):1311-1317. (Biology). View Reference
      7. Renauld JC. New insights into the role of cytokines in asthma. J Clin Pathol. 2001 August; 54(8):577-589. (Biology). View Reference
      8. Soler D, Chapman TR, Poisson LR. CCR8 expression identifies CD4 memory T cells enriched for FOXP3+ regulatory and Th2 effector lymphocytes. J Immunol. 2006; 177(10):6940-6951. (Biology). View Reference
      9. Soroosh P, Doherty TA. Th9 and allergic disease. Immunology. 2009; 127(4):450-458. (Biology). View Reference
      View All (9) View Less

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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