Multicolor flow cytometric analysis of Ly-108 expression on mouse bone marrow cells and thymocytes. Mouse bone marrow cells and thymocytes were stained with Alexa Fluor® 647 Mouse anti-Mouse Ly-108, PE Rat anti-Mouse CD45R/B220 (Cat. No. 553090) and BD Horizon™ V450 Hamster Anti-Mouse CD3e (Cat. No. 560801) antibodies. Two-color flow cytometric dot plots show the correlated expression of CD45R (bone marrow cells, Left Panel) or CD3 (thymocytes, Right Panel) versus Ly-108 for gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
Multicolor flow cytometric analysis of Ly-108 expression on mouse bone marrow cells and thymocytes. Mouse bone marrow cells and thymocytes were stained with Alexa Fluor® 647 Mouse anti-Mouse Ly-108, PE Rat anti-Mouse CD45R/B220 (Cat. No. 553090) and BD Horizon™ V450 Hamster Anti-Mouse CD3e (Cat. No. 560801) antibodies. Two-color flow cytometric dot plots show the correlated expression of CD45R (bone marrow cells, Left Panel) or CD3 (thymocytes, Right Panel) versus Ly-108 for gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
Product Details
BD Pharmingen™
CD352; Slamf6; SLAM family member 6; KAL1; NTB-A; SF2000
Mouse (QC Testing)
Mouse IgG2a, κ
WT thymocytes
Flow cytometry (Routinely Tested)
0.2 mg/ml
30925
AB_10712759
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
PE Rat Anti-Mouse CD45R/B220 RUO
Size
0.2 mg
Cat No.
553090
V450 Hamster anti-Mouse CD3e RUO
Size
0.1 mg
Cat No.
560801
561547 Rev. 1
Antibody Details
13G3
The 13G3 monoclonal antibody specifically binds to Lymphocyte antigen 108, Ly-108. Ly-108 is a member of the signaling lymphocytic activation molecule (SLAM) family of immune receptors. Ly-108 is a type 1 transmembrane glycoprotein adhesion receptor that is expressed by T cells, NKT cells, B cells, NK cells, macrophages, dendritic cells and neutrophils. Ly-108 plays multiple roles in innate and adaptive immunity including costimulation of NK cell cytotoxicity and T cell cytokine responses. Moreover, Ly-108 has been implicated in autoimmunity.
561547 Rev. 1
Format Details
Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
561547 Rev.1
Citations & References
Development References (4)
-
Griewank K, C Borowski, Rietdijk S, et al. Homotypic interactions mediated by Slamf1 and Slamf6 receptors control NKT cell lineage development. Immunity. 2007; 27(5):751-762. (Clone-specific: Flow cytometry, Immunofluorescence). View Reference
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Howie D, Laroux FS, Morra M, et al. Cutting edge: the SLAM family receptor Ly108 controls T cell and neutrophil functions. J Immunol. 2005; 10(174):5931-5935. (Biology). View Reference
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Li W, Sofi MH, Rietdijk S, Wang N, et al. The SLAM-Associated Protein (SAP)/Fyn/PKCθ Pathway is Required for Thymocyte-mediated CD4 T Cell Development. Immunity. 2007; 27(2):763-774. (Biology). View Reference
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Peck SR, Ruley HE. Ly108: a new member of the mouse CD2 family of cell surface proteins. J Immunol. 2000; 52(1-2):63-72. (Biology). View Reference
561547 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
