AmCyan Mouse Anti-Human CD4
Clone SK3 (also known as Leu3a) (RUO (GMP))
- Concentration 6.0 μg/mL
- Vol. Per Test 5 μL
- Isotype Mouse BALB/c IgG1, κ
- Reactivity Human (QC Testing)
Flow cytometry (Routinely Tested)
- Immunogen Human Peripheral Blood T Cells
- Workshop No. I T38; III T140,496
- Entrez Gene ID 920
- Storage Buffer Phosphate buffered saline with BSA and 0.1% sodium azide.
- Regulatory Status RUO (GMP)
Regulatory Status Legend
The CD4 antibody, clone SK3, is derived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human peripheral blood T lymphocytes.
The CD4 antibody recognizes a 55-kilodalton (kDa) glycoprotein that is present on T-helper/inducer lymphocytes and monocytes.
AmCyan is a 108-kDa protein derived from Anemonia majano. With an excitation peak of 457 nm and an emission peak of 491 nm, it can be used on violet laser–equipped flow cytometers in combination with BD Horizon BV421, BD Horizon V450, or Pacific Blue™, but not BD Horizon™ V500.
Preparation and Storage
Store vials at 2°C to 8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.