AmCyan Mouse Anti-Human CD3
Clone SK7 (also known as Leu-4) (RUO (GMP))
- Concentration 100.0 μg/mL
- Vol. Per Test 5 μL
- Isotype Mouse BALB/c IgG1, κ
- Reactivity Human (QC Testing)
Flow cytometry (Routinely Tested)
- Immunogen Human Thymocytes
- Workshop No. II T118; III T492
- Entrez Gene ID 916
- Storage Buffer Phosphate buffered saline with BSA and 0.1% sodium azide.
- Regulatory Status RUO (GMP)
Regulatory Status Legend
CD3 clone SK7 is derived from hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human thymocytes.
The CD3 antibody reacts with the epsilon chain of the CD3 antigen/T-cell antigen receptor (TCR) complex. This complex is composed of at least six proteins that range in molecular weight from 20 to 30 kilodaltons (kDa). The antigen recognized by CD3 antibodies is noncovalently associated with either α/β or γ/δ TCR (70 to 90 kDa).
AmCyan is a 108-kDa protein derived from Anemonia majano. With an excitation peak of 457 nm and an emission peak of 491 nm, it can be used on violet laser–equipped flow cytometers in combination with BD Horizon BV421, BD Horizon V450, or Pacific Blue™, but not BD Horizon™ V500.
Preparation and Storage
Store vials at 2°C to 8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.