Purified Mouse Anti-Human CD54
Clone 28/CD54 (RUO)
- Brand BD Transduction Laboratories™
- Alternative Name ICAM-1
- Concentration 250 µg/ml
- Isotype Mouse IgG1
- Reactivity Human (QC Testing)
Western blot (Routinely Tested)
Fluorescence microscopy (Not Recommended)
- Immunogen Human CD54/ICAM-1 aa. 46-160
- Storage Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
The migration of T cells through lymph nodes and their interactions with antigen presenting cells (APCs) involve adhesion molecules on the surfaces of the T cell and the cell with which it interacts. These molecules include the selectins, the integrins, members of the immunoglobulin superfamily, and some mucin-like proteins. The integrins mediate adhesion between cells and between cells and the extracellular matrix. LFA-1 (lymphocyte function-associated antigen 1), a member of the ß2 integrin family, is expressed on T cells, neutrophils, and macrophages and is thought to be the most important adhesion molecule for lymphocyte activation. It interacts with members of the immunoglobulin superfamily, the intercellular adhesion molecules: ICAM-1, -2, and -3. ICAM-1 and ICAM-2 are expressed on endodthelium and APCs, while ICAM-3 is expressed only on leukocytes. ICAM-1 is most homologous to neural cell adhesion molecule and myelin-associated glycoprotein. It is a 55 kDa glycoprotein that is heavily glycosylated to form 90 kDa to 115 kDa proteins. Its interactions with LFA-1 are necessary for CTL and NK mediated killing, T and B cell responses, and immune cell extravasation.
- Format Purified
Preparation and Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Store undiluted at -20°C.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.