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Western blot analysis of CD95 on a Daudi cell lysate (Human B lymphoblast; ATCC CCL-213). Lane 1:5,000, lane 2: 1:10,000, lane 3: 1:20,000 in dilution of thePurified Mouse anti-CD95 antibody.
Immunohistochemical staining of a formalin-fixed rabbit liver section.
BD Transduction Laboratories™ Purified Mouse Anti-CD95
BD Transduction Laboratories™ Purified Mouse Anti-CD95
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Recommended Assay Procedures
For Western Blot and immunohistochemistry resources, please reference http://www.bdbiosciences.com/resources/cellbiology/index.jsp
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
CD95 is a member of a family of cell surface receptors that includes tumor necrosis factor receptor (TNF-R), nerve growth factor receptor (NGF-R), CD40, CD27, CD30, and 4-1BB. Both murine and human Fas genes have been cloned and reportedly share 60% similarity in their amino acid sequences. CD95 (Fas) is a cell surface apoptosis-signaling molecule that is widely expressed in sites such as thymus, liver, heart, and ovary. Abnormalities in the Fas gene correlate with autoimmune features in mice and with unusually high levels of lymphocyte apoptosis in HIV-infected humans. Genetic studies have localized the Fas gene near the lpr (lymphoproliferation disease) locus on mouse chromosome 19 and further characterization reportedly has revealed that lpr is a mutation affecting the function of the Fas gene.
Development References (5)
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Arnold R, Seifert M, Asadullah K, Volk HD. Crosstalk between keratinocytes and T lymphocytes via Fas/Fas ligand interaction: modulation by cytokines.. J Immunol. 1999; 162(12):7140-7147. (Biology: Flow cytometry). View Reference
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Itoh N, Yonehara S, Ishii A, et al. The polypeptide encoded by the cDNA for human cell surface antigen Fas can mediate apoptosis. Cell. 1991; 66(2):233-243. (Biology). View Reference
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MacLaren A, Clark W, Gillespie DA. v-Jun sensitizes cells to apoptosis by a mechanism involving mitochondrial cytochrome C release.. Oncogene. 2000 ; 19(51):5906-5918. (Biology: Western blot). View Reference
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Rosen K, Coll ML, Li A, Filmus J. Transforming growth factor-alpha prevents detachment-induced inhibition of c-Src kinase activity, Bcl-XL down-regulation, and apoptosis of intestinal epithelial cells.. 2001; 276(40):37273-37279. (Clone-specific: Western blot). View Reference
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Zhuang S, Demirs JT, Kochevar IE. Protein kinase C inhibits singlet oxygen-induced apoptosis by decreasing caspase-8 activation.. Oncogene. 2001; 20(46):6764-6776. (Biology: Immunofluorescence, Immunoprecipitation, Western blot). View Reference
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