Purified Mouse Anti-CD95
Clone 13/Fas (RUO)
- Brand BD Transduction Laboratories™
- Alternative Name Fas, APO-1
- Concentration 250 µg/ml
- Isotype Mouse IgG2a, κ
- Reactivity Human (QC Testing) Mouse, Rat, Dog, Chicken (Tested in Development)
Western blot (Routinely Tested)
Immunohistochemistry-formalin (antigen retrieval required) (Tested During Development)
Immunofluorescence, Immunoprecipitation (Not Recommended)
- Immunogen Human Fas aa. 1-163
- Storage Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
CD95 is a member of a family of cell surface receptors that includes tumor necrosis factor receptor (TNF-R), nerve growth factor receptor (NGF-R), CD40, CD27, CD30, and 4-1BB. Both murine and human Fas genes have been cloned and reportedly share 60% similarity in their amino acid sequences. CD95 (Fas) is a cell surface apoptosis-signaling molecule that is widely expressed in sites such as thymus, liver, heart, and ovary. Abnormalities in the Fas gene correlate with autoimmune features in mice and with unusually high levels of lymphocyte apoptosis in HIV-infected humans. Genetic studies have localized the Fas gene near the lpr (lymphoproliferation disease) locus on mouse chromosome 19 and further characterization reportedly has revealed that lpr is a mutation affecting the function of the Fas gene.
- Format Purified
Suggested Companion Products
Preparation and Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Store undiluted at -20°C.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
For Western Blot and immunohistochemistry resources, please reference http://www.bdbiosciences.com/resources/cellbiology/index.jsp