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APC Mouse Anti-Human CD2 L303.1 (also known as L303) RUO (GMP)

APC Mouse Anti-Human CD2 

Clone  L303.1 (also known as L303)   (RUO (GMP))

  • Concentration 50 μg/mL
  • Vol. Per Test 5 μL
  • Isotype Mouse BALB/c IgG2a, κ
  • Reactivity Human (QC Testing) 
  • Application Flow cytometry (Routinely Tested) 
  • Immunogen CD3-activated Human T Lymphocytes
  • Storage Buffer Phosphate buffered saline with gelatin and 0.1% sodium azide.
  • Regulatory Status RUO (GMP)
Product Details Recommended Assay References

Description

• CD2, clone S5.2, is derived from hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with T lymphocytes activated by mixed lymphocyte culture.

• CD2, clone L303.1, is derived by the fusion of Sp2/0 myeloma cells with splenocytes from BALB/c mice immunized with CD3-activated human T lymphocytes.

CD2 recognizes a human lymphocyte antigen, 45 to 50 kilodaltons (kDa), which also forms the binding site for sheep erythrocytes.

Format
  • Format APC
  • Excitation Source Red 633 nm
  • Excitation Max 650 nm
  • Emission Max 660 nm

Allophycocyanin (APC), is an accessory photosynthetic pigment found in blue-green algae. Its molecular weight is approximately 105 kDa. APC has six phycocyanobilin chromophores per molecule, which makes it a very bright fluorochrome that is highly suitable for flow cytometry applications. Due to nearly identical excitation and emission properties but different spillover characteristics, APC and Alexa Fluor® 647 cannot be used simultaneously

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Preparation and Storage

Store vials at 2°C to 8°C. Conjugated forms should not be frozen and should be protected from exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.


  1. Bieber CP, Howard FD, Pennock J, Wong J, Shorthouse R, Stinson EB. Preparation, characterization, and primate testing of monoclonal antithymocyte globulin. Transplantation. 1981;31:283.

  2. Bierer BE, Peterson A, Gorga JC, Herrmann SH, Burakoff SJ. Synergistic T-cell activation via the physiological ligands for CD2 and the T-cell receptor. J Exp Med. 1988;168:1145-1156.

  3. Crawford K, Gabuzda D, Pantazopoulos V, et al. Circulating CD2+ monocytes are dendritic cells. J Immunol. 1999;163:5920-5928.

  4. Haynes BF. Summary of T-cell studies performed during the Second International Workshop and Conference on Human Leukocyte Differentiation Antigens. In: Reinherz EL, Haynes BF, Nadler LM, Bernstein ID, eds. Leukocyte Typing II: Human T Lymphocytes. New York, NY: Springer-Verlag; 1986;1:3-30.

  5. Howard FD, Ledbetter JA, Wong J, Bieber CP, Stinson EB, Herzenberg LA. A human T lymphocyte differentiation marker defined by monoclonal antibodies that block E-rosette formation. J Immunol. 1981;126:2117-2122.

  6. Koyasu S, Lawton T, Novick D, et al. Role of interaction of CD2 molecules with lymphocyte functionassociated antigen 3 in T-cell recognition of nominal antigen. Proc Natl Acad Sci USA. 1990;87:2603-2607.

  7. Lanier LL, Phillips JH. A map of the cell surface antigens expressed on resting and activated human natural killer cells. In: Reinherz EL, Haynes BF, Nadler LM, Bernstein ID, eds. Leukocyte Typing II: Human Myeloid and Hematopoietic Cells. New York, NY: Springer-Verlag; 1986;3:157-170.

  8. MacDonald KPA, Munster DJ, Clark GJ, Dzionek A, Schmitz J, Hart DNJ. Characterization of human blood dendritic cell subsets. Blood. 2002;100:4512-4520.

  9. Moingeon P, Chang H, Wallner BP, Stebbins C, Frey AZ, Reinherz EL. CD2-mediated adhesion facilitates T-lymphocyte antigen-recognition function. Nature. 1989;339:312-314.

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