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      Alexa Fluor® 488 Mouse Anti-Human TGF-β1
      Alexa Fluor® 488 Mouse Anti-Human TGF-β1
      Flow cytometric analysis of human TGF-β1 expressed by TGF-β1-transfected P3UI cells. Untransfected mouse P3UI myeloma cells (dashed line histogram) and human TGF-β1-transfected P3UI cells (solid line histogram) were fixed and permeabilized for 30 minutes with BD Cytofix/Cytoperm™ Fixation and Permeabilization Solution (Cat. No. 554722) and washed with BD Perm/Wash™ Perm/Wash Buffer (Cat. No. 554723). The cells were then stained with Alexa Fluor® 488 Mouse Anti-Human TGF-β1 antibody (Cat. No. 562545). The flow cytometric fluorescence histograms were derived from gated events with the forward- and side light-scattering characteristics of intact cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
      Alexa Fluor® 488 Mouse Anti-Human TGF-β1
      Flow cytometric analysis of human TGF-β1 expressed by TGF-β1-transfected P3UI cells. Untransfected mouse P3UI myeloma cells (dashed line histogram) and human TGF-β1-transfected P3UI cells (solid line histogram) were fixed and permeabilized for 30 minutes with BD Cytofix/Cytoperm™ Fixation and Permeabilization Solution (Cat. No. 554722) and washed with BD Perm/Wash™ Perm/Wash Buffer (Cat. No. 554723). The cells were then stained with Alexa Fluor® 488 Mouse Anti-Human TGF-β1 antibody (Cat. No. 562545). The flow cytometric fluorescence histograms were derived from gated events with the forward- and side light-scattering characteristics of intact cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
      Product Details
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      BD Pharmingen™
      TGFB1; TGFbeta1; TGF-beta-1; Transforming growth factor, beta 1; CED; LAP
      Human (QC Testing)
      Mouse BALB/c IgG1, κ
      Human TGF-β1 Transfected Cell Line
      Intracellular staining (flow cytometry) (Routinely Tested)
      5 µl
      AB_2737645
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed.
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      Product Notices

      1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      4. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
      5. Alexa Fluor® 488 fluorochrome emission is collected at the same instrument settings as for fluorescein isothiocyanate (FITC).
      6. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
      7. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      8. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
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      562545 Rev. 1
      Antibody Details
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      TW4-9E7

      The TW4-9E7 monoclonal antibody specifically binds to Human Transforming Growth Factor beta-1 (TGF-β1). TGF-β1 is a potent multifunctional cytokine that positively or negatively regulates numerous processes including development, hematopoiesis, tissue remodeling, wound repair, innate and adaptive immunity as well as cancer and autoimmune diseases. TGF-β1 is formed by the enzymatic cleavage of the TGF-β1 propeptide that is encoded by the TGFB1 gene and comprised of the Latency Associated Peptide (LAP) and TGF-β1. Prior to secretion, the dimeric LAP-TGF-β1 propeptide is cleaved resulting in a biologically inactive form of dimeric TGF-β1 that is noncovalently associated with dimeric LAP (latent TGF-β1). This complex may be expressed on the surface of TGF-β1-producing cells or be further processed by proteolytic removal of LAP to release the biologically active mature form of the soluble TGF-β1 homodimer. Many different cell types synthesize TGF-β1 and express specific receptors for it. The TW4-9E7 antibody recognizes both the intracellular latent bound form of TGF-β1 along with the membrane bound form of TGF-β1.

        

      562545 Rev. 1
      Format Details
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      Alexa Fluor™ 488
      Alexa Fluor™ 488 Dye is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 517-nm. Alexa Fluor™ 488 is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 520-nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      Alexa Fluor™ 488
      Blue 488 nm
      494 nm
      517 nm
      562545 Rev.1
      Citations & References
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      Development References (6)

      1. Derynck R, Akhurst RJ. Differentiation plasticity regulated by TGF-beta family proteins in development and disease. Nat Cell Biol. 2007; 9(9):1000-1004. (Biology). View Reference
      2. Derynck R, Jarrett JA, Chen EY, et al. Human transforming growth factor-beta complementary DNA sequence and expression in normal and transformed cells. Nature. 1985; 316(6030):701-705. (Biology). View Reference
      3. Dünker N, Krieglstein K. Targeted mutations of transforming growth factor-beta genes reveal important roles in mouse development and adult homeostasis. Eur J Biochem. 2000; 267(24):6982-6988. (Biology). View Reference
      4. Miyazono K, Hellman U, Wernstedt C, Heldin CH. Latent high molecular weight complex of transforming growth factor beta 1. Purification from human platelets and structural characterization. J Biol Chem. 1988; 263(13):6407-6415. (Biology). View Reference
      5. Oida T, Weiner HL. Overexpression of TGF-β1 gene induces cell surface localized glucose-regulated protein 78-associated latency-associated peptide/TGF-β. J Immunol. 2010; 185(6):3529-3535. (Immunogen: Flow cytometry). View Reference
      6. Rubtsov YP, Rudensky AY. TGFbeta signalling in control of T-cell-mediated self-reactivity. Nat Rev Immunol. 2007; 7(6):443-453. (Biology). View Reference
      View All (6) View Less
      562545 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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