PerCP-Cy™5.5 Mouse Lineage Antibody Cocktail, with Isotype Control
- Brand BD Pharmingen™
- Reactivity Mouse (QC Testing)
Flow cytometry (Routinely Tested)
- Regulatory Status RUO
Regulatory Status Legend
The PerCP-Cy™5.5 Mouse Lineage Antibody Cocktail has been designed to react with cells from the major hematopoietic lineages, such as T lymphocytes, B lymphocytes, monocytes/macrophages, NK cells, erythrocytes, and granulocytes. This pre-diluted Cocktail of five PerCP-Cy™5.5-conjugated antibodies is designed to label lineage marker-positive cells for exclusion to facilitate the flow cytometric identification of lineage marker-negative hematopoietic progenitors in mouse bone marrow. Components include clone 145-2C11, which recognizes Mouse CD3e; M1/70, which recognizes CD11b; RA3-6B2, which recognizes CD45R/B220; TER-119, which recognizes Ly-76, mouse erythroid cells; and RB6-8C5, which recognizes Ly-6G and Ly-6C. PerCP-Cy™5.5 Mouse Lineage Isotype Control Cocktail contains equivalent concentrations of isotype-matched negative-control immunoglobulin. Additional fluorochrome-labeled reagents may be combined with the PerCP-Cy™5.5 Mouse Lineage Antibody Cocktail, and the PerCP-Cy™5.5 Mouse Lineage Isotype Control Cocktail, to further characterize hematopoietic progenitor subpopulations.
PerCP-Cy™5.5 Mouse Lineage Antibody Cocktail
||100 Tests (1 ea)|
PerCP-Cy™5.5 Mouse Lineage Isotype Control Cocktail
||100 Tests (1 ea)|
Suggested Companion Products
Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
The antibody was conjugated with PerCP-Cy5.5 under optimum conditions, and unconjugated antibody and free PerCP-Cy5.5 were removed. Storage of PerCP-Cy5.5 conjugates in unoptimized diluent is not recommended and may result in loss of signal intensity.
20 µl of the antibody or isotype control cocktail is sufficient to stain each sample of 10^6 leukocytes for flow cytometric analysis. We recommend the use of Mouse BD Fc Block™ purified anti-mouse CD16/Cd32 mAb 2.4G2 (Cat. No. 553141/553142) for optimal staining. The PerCP-Cy™5.5 Mouse Lineage Antibody Cocktail can be used to deplete cells bearing the hematopoietic lineage markers by flow cytometric sorting. The PerCP-Cy™5.5 fluorochrome is excited by laser lines from 595 to 647 nm, and its emission is collected in a detector for fluorescence wavelengths between 640 and 680 nm.