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Purified NA/LE Rat Anti-CD11b M1/70 RUO

Purified NA/LE Rat Anti-CD11b 

Clone  M1/70   (RUO)

  • Brand BD Pharmingen™
  • Alternative Name Itgam; Integrin alpha-M; Ly-40; Mac-1a; Mac-1 alpha; CR3A; CR-3 alpha chain
  • Concentration 1.0 mg/ml
  • Isotype Rat DA, also known as DA/HA IgG2b, κ
  • Reactivity Mouse (QC Testing)  Human (Tested in Development) 
  • Application Flow cytometry (Routinely Tested) 
    Immunoprecipitation, Blocking, Immunohistochemistry-frozen (Reported) 
    Western blot, Immunohistochemistry-paraffin (Not Recommended) 
  • Immunogen Mouse Splenic Cells
  • Entrez Gene ID 16409 
  • Storage Buffer No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
  • Regulatory Status RUO
Product Details Recommended Assay References

Description

The M1/70 monoclonal antibody specifically binds to CD11b, also known as Integrin alpha M (Itgam or αM). CD11b is a 170-kDa type 1 transmembrane glycoprotein and belongs to the Integrin alpha chain family. CD11b serves as the alpha chain of the heterodimeric Mac-1 integrin (CD11b/CD18, αMβ2), also known as complement receptor 3 (CR3). Mac-1 mediates adhesion to ICAM-1 (CD54), ICAM-2 (CD102), fibrinogen and binding to C3bi. Mac-1 is expressed at varying levels on granulocytes, macrophages, myeloid-derived dendritic cells, natural killer cells, microglia, and B-1 B lymphocytes. Mac-1 expression is rapidly upregulated on neutrophils after activation, in the same time period that CD62L (L-selectin) is shed from the cell surface. The M1/70 antibody reportedly blocks cell adherence and C3bi binding but does not block cell-mediated lysis. Cross-reaction of the M1/70 antibody with CD11b expressed on human monocytes, polymorphonuclear leukocytes, and NK cells has been reported.

Format
  • Format NA/LE

Other Formats →

Suggested Companion Products

Purified NA/LE Rat IgG2b, κ Isotype Control RUO
0.5 mg Cat No: 553985

Purified Rat Anti-CD11b M1/70 RUO
0.5 mg Cat No: 553308

FITC Goat Anti-Rat Ig Polyclonal RUO
0.5 mg Cat No: 554016

Lysing Buffer RUO
100 mL Cat No: 555899

Stain Buffer (FBS) RUO
500 mL Cat No: 554656

Stain Buffer (BSA) RUO
500 mL Cat No: 554657

Resources & Tools
 Spectrum Viewer  Panel Designer  Spectrum Viewer  Download TDS  Regulatory Document Website
Preparation and Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
  4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.


  1. Ault KA, Springer TA. Cross-reaction of a rat-anti-mouse phagocyte-specific monoclonal antibody (anti-Mac-1) with human monocytes and natural killer cells. J Immunol. 1981; 126(1):359-364. (Clone-specific: Immunohistochemistry-paraffin, Western blot, Immunohistochemistry-frozen, Immunoprecipitation, Blocking, Flow cytometry). View reference

  2. Beller DI, Springer TA, Schreiber RD. Anti-Mac-1 selectively inhibits the mouse and human type three complement receptor. J Exp Med. 1982; 156(4):1000-1009. (Biology: Immunohistochemistry-paraffin, Western blot, Immunohistochemistry-frozen, Immunoprecipitation, Blocking, Flow cytometry). View reference

  3. Flotte TJ, Springer TA, Thorbecke GJ. Dendritic cell and macrophage staining by monoclonal antibodies in tissue sections and epidermal sheets. Am J Pathol. 1983; 111(1):112-124. (Clone-specific: Immunohistochemistry-paraffin, Western blot, Immunohistochemistry-frozen, Immunoprecipitation, Blocking, Flow cytometry). View reference

  4. Gyetko MR, Sud S, Kendall T, Fuller JA, Newstead MW, Standiford TJ. Urokinase receptor-deficient mice have impaired neutrophil recruitment in response to pulmonary Pseudomonas aeruginosa infection. J Immunol. 2000; 165(3):1513-1519. (Biology: Immunohistochemistry-paraffin, Western blot, Immunohistochemistry-frozen, Immunoprecipitation, Blocking, Flow cytometry). View reference

  5. Kishimoto TK, Jutila MA, Berg EL, Butcher EC. Neutrophil Mac-1 and MEL-14 adhesion proteins inversely regulated by chemotactic factors. Science. 1989; 245(4923):1238-1241. (Biology: Immunohistochemistry-paraffin, Western blot, Immunohistochemistry-frozen, Immunoprecipitation, Blocking, Flow cytometry). View reference

  6. Lagasse E, Weissman IL. Flow cytometric identification of murine neutrophils and monocytes. J Immunol Methods. 1996; 197(1-2):139-150. (Methodology: Immunohistochemistry-paraffin, Western blot, Immunohistochemistry-frozen, Immunoprecipitation, Blocking, Flow cytometry). View reference

  7. Lub M, van Kooyk Y, Figdor CG. Competition between lymphocyte function-associated antigen 1 (CD11a/CD18) and Mac-1 (CD11b/CD18) for binding to intercellular adhesion molecule-1 (CD54). J Leukoc Biol. 1996; 59(5):648-655. (Biology: Immunohistochemistry-paraffin, Western blot, Immunohistochemistry-frozen, Immunoprecipitation, Blocking, Flow cytometry). View reference

  8. Sanchez-Madrid F, Simon P, Thompson S, Springer TA. Mapping of antigenic and functional epitopes on the alpha- and beta-subunits of two related mouse glycoproteins involved in cell interactions, LFA-1 and Mac-1. J Exp Med. 1983; 158(2):586-602. (Biology: Immunohistochemistry-paraffin, Western blot, Immunohistochemistry-frozen, Immunoprecipitation, Blocking, Flow cytometry). View reference

  9. Springer T, Galfre G, Secher DS, Milstein C. Mac-1: a macrophage differentiation antigen identified by monoclonal antibody. Eur J Immunol. 1979; 9(4):301-306. (Clone-specific: Immunohistochemistry-paraffin, Western blot, Immunohistochemistry-frozen, Immunoprecipitation, Blocking, Flow cytometry). View reference

  10. Springer T, Galfre G, Secher DS, Milstein C. Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens. Eur J Immunol. 1978; 8(8):539-551. (Immunogen: Immunohistochemistry-paraffin, Western blot, Immunohistochemistry-frozen, Immunoprecipitation, Blocking, Flow cytometry). View reference

  11. Springer TA, Davignon D, Ho MK, Kurzinger K, Martz E, Sanchez-Madrid F. LFA-1 and Lyt-2,3, molecules associated with T lymphocyte-mediated killing; and Mac-1, an LFA-1 homologue associated with complement receptor function. Immunol Rev. 1982; 68:171-195. (Biology: Immunohistochemistry-paraffin, Western blot, Immunohistochemistry-frozen, Immunoprecipitation, Blocking, Flow cytometry). View reference

553307 Rev. 20
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