Purified NA/LE Rat Anti-CD11b
Clone M1/70 (RUO)
- Brand BD Pharmingen™
- Alternative Name Itgam; Integrin alpha-M; Ly-40; Mac-1a; Mac-1 alpha; CR3A; CR-3 alpha chain
- Concentration 1.0 mg/ml
- Isotype Rat DA, also known as DA/HA IgG2b, κ
- Reactivity Mouse (QC Testing) Human (Tested in Development)
- Application
Flow cytometry (Routinely Tested)
Immunoprecipitation, Blocking, Immunohistochemistry-frozen (Reported)
Western blot, Immunohistochemistry-paraffin (Not Recommended)
- Immunogen Mouse Splenic Cells
- Entrez Gene ID 16409
- Storage Buffer No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
- Regulatory Status RUO
Regulatory Status Legend
Description
The M1/70 monoclonal antibody specifically binds to CD11b, also known as Integrin alpha M (Itgam or αM). CD11b is a 170-kDa type 1 transmembrane glycoprotein and belongs to the Integrin alpha chain family. CD11b serves as the alpha chain of the heterodimeric Mac-1 integrin (CD11b/CD18, αMβ2), also known as complement receptor 3 (CR3). Mac-1 mediates adhesion to ICAM-1 (CD54), ICAM-2 (CD102), fibrinogen and binding to C3bi. Mac-1 is expressed at varying levels on granulocytes, macrophages, myeloid-derived dendritic cells, natural killer cells, microglia, and B-1 B lymphocytes. Mac-1 expression is rapidly upregulated on neutrophils after activation, in the same time period that CD62L (L-selectin) is shed from the cell surface. The M1/70 antibody reportedly blocks cell adherence and C3bi binding but does not block cell-mediated lysis. Cross-reaction of the M1/70 antibody with CD11b expressed on human monocytes, polymorphonuclear leukocytes, and NK cells has been reported.
Format
Suggested Companion Products
Purified NA/LE Rat IgG2b, κ Isotype Control RUO
0.5 mg
Cat No: 553985
Purified Rat Anti-CD11b M1/70 RUO
0.5 mg
Cat No: 553308
FITC Goat Anti-Rat Ig Polyclonal RUO
0.5 mg
Cat No: 554016
Lysing Buffer RUO
100 mL
Cat No: 555899
Stain Buffer (FBS) RUO
500 mL
Cat No: 554656
Stain Buffer (BSA) RUO
500 mL
Cat No: 554657
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Preparation and Storage
Store undiluted at 4°C.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.