V450 Mouse Anti-Human CD271
Clone C40-1457 (RUO)
- Brand BD Horizon™
- Alternative Name NGFR; NGF Receptor; TNFRSF16
- Vol. Per Test 5 µl
- Isotype Mouse IgG1, κ
- Reactivity Human (QC Testing)
Flow cytometry (Routinely Tested)
- Immunogen Human NGFR Recombinant Protein
- Workshop No. VIII 80150
- Storage Buffer Aqueous buffered solution containing protein stabilizer, glycerol and ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
The C40-1457 monoclonal antibody specifically recognizes CD271 that is also known as the nerve growth factor receptor (NGFR). CD271 is 75 kDa type I transmembrane glycoprotein likewise known as TNFRSF16 that belongs to the tumor necrosis factor receptor (TNFR) superfamily. CD271 has been found localized to neuronal axons, Schwann cells, and perineural cells of peripheral nerves. It is also expressed by some epithelial, mesenchymal and lymphoid tissues. NGFR is the receptor for nerve growth factor (NGF), a polypeptide that is essential for normal development of the nervous system. NGF promotes survival and differentiation of sympathetic and sensory neurons during embryological development of the peripheral nervous system. NGF binds to two distinctive surface receptors, the p/140[prototrk] and p75[NGFR]. High affinity binding of NGF requires that both receptor molecules be expressed. NGFR is expressed on human and rat lymphocytes. A subset of lymphoid cells in the spleen, lymph nodes, and follicular dendritic cells in germinal centers of reactive lymph nodes were found to express CD271. It has been reported that NGFR interaction with its ligand, NGF, may play a role in immunoregulation. NGF may also function as a B-cell growth factor.
The antibody is conjugated to BD Horizon™ V450, which has been developed for use in multicolor flow cytometry experiments and is available exclusively from BD Biosciences. It is excited by the Violet laser Ex max of 406 nm and has an Em Max at 450 nm. Conjugates with BD Horizon™ V450 can be used in place of Pacific Blue™ conjugates.
BD Horizon™ V450 is a coumarin dye excited by the violet laser that exhibits spectral properties similar to Pacific Blue™. Conjugates are typically as bright or brighter than comparable reagents conjugated to Pacific Blue™. Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon V450 cannot be used simultaneously.
Suggested Companion Products
Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated with BD Horizon™ V450 under optimum conditions, and unreacted BD Horizon™ V450 was removed.
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- BD Horizon V450 has a maximum absorption of 406 nm and maximum emission of 450 nm. Before staining with this reagent, please confirm that your flow cytometer is capable of exciting the fluorochrome and discriminating the resulting fluorescence.
- Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.