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![PE-Cy™7 Mouse Anti-Human CD14](/content/dam/bdb/products/global/reagents/flow-cytometry-reagents/research-reagents/single-color-antibodies-ruo/561xxx/5613xx/561385_base/NHP_CD14_PE-Cy7_overlay.png)
![PE-Cy™7 Mouse Anti-Human CD14](/content/dam/bdb/products/global/reagents/flow-cytometry-reagents/research-reagents/single-color-antibodies-ruo/561xxx/5613xx/561385_base/NHP_CD14_PE-Cy7_overlay.png)
![](/content/dam/bdb/products/vialimages/packaging-images/black-vial-images/Blue Cap - Blue Label (Transparent).png)
![PE-Cy™7 Mouse Anti-Human CD14](/content/dam/bdb/products/global/reagents/flow-cytometry-reagents/research-reagents/single-color-antibodies-ruo/561xxx/5613xx/561385_base/NHP_CD14_PE-Cy7_overlay.png)
Flow cytometric analysis of CD14 expression on Rhesus macaque peripheral blood monocytes. Rhesus macaque whole blood was stained with the PE-Cy™7 Mouse anti-Human CD14 antibody (Cat. No. 561385; solid line histogram) or with a PE-Cy™7 Mouse IgG2a, κ Isotype Control (Cat. No. 552868; dashed line histogram). The erythrocytes were lysed with BD PharmLyse™ Lysing Buffer (Cat. No. 555899). The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable monocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
![](/content/dam/bdb/products/vialimages/packaging-images/black-vial-images/Blue Cap - Blue Label (Transparent).png)
![PE-Cy™7 Mouse Anti-Human CD14](/content/dam/bdb/products/global/reagents/flow-cytometry-reagents/research-reagents/single-color-antibodies-ruo/561xxx/5613xx/561385_base/NHP_CD14_PE-Cy7_overlay.png)
BD Pharmingen™ PE-Cy™7 Mouse Anti-Human CD14
![](/content/dam/bdb/products/vialimages/packaging-images/black-vial-images/Blue Cap - Blue Label (Transparent).png)
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Cy is a trademark of Amersham Biosciences Limited. This conjugated product is sold under license to the following patents: US Patent Nos. 5,486,616; 5,569,587; 5,569,766; 5,627,027.
- Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Warning: Some APC-Cy7 and PE-Cy7 conjugates show changes in their emission spectrum with prolonged exposure to formaldehyde. If you are unable to analyze fixed samples within four hours, we recommend that you use BD™ Stabilizing Fixative (Cat. No. 338036).
- This product is subject to proprietary rights of Amersham Biosciences Corp. and Carnegie Mellon University and made and sold under license from Amersham Biosciences Corp. This product is licensed for sale only for research. It is not licensed for any other use. If you require a commercial license to use this product and do not have one return this material, unopened to BD Biosciences, 10975 Torreyana Rd, San Diego, CA 92121 and any money paid for the material will be refunded.
- PE-Cy7 is a tandem fluorochrome composed of R-phycoerythrin (PE), which is excited by 488-nm light and serves as an energy donor, coupled to the cyanine dye Cy7, which acts as an energy acceptor and fluoresces maximally at 780 nm. PE-Cy7 tandem fluorochrome emission is collected in a detector for fluorescence wavelengths of 750 nm and higher. Although every effort is made to minimize the lot-to-lot variation in the efficiency of the fluorochrome energy transfer, differences in the residual emission from PE may be observed. Therefore, we recommend that individual compensation controls be performed for every PE-Cy7 conjugate. PE-Cy7 is optimized for use with a single argon ion laser emitting 488-nm light, and there is no significant overlap between PE-Cy7 and FITC emission spectra. When using dual-laser cytometers, which may directly excite both PE and Cy7, we recommend the use of cross-beam compensation during data acquisition or software compensation during data analysis.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
![sampleImage/](/content/dam/bdb/products/global/reagents/cell-preparation-separation-reagents/blood-lysis/staining-and-cell-preparation/554xxx/5546xx/554656_base/554656_01.png?imwidth=320)
![sampleImage/](/content/dam/bdb/products/global/reagents/cell-preparation-separation-reagents/blood-lysis/staining-and-cell-preparation/555xxx/5558xx/555899_base/35221E_555899_Figure.png?imwidth=320)
![sampleImage/](/content/dam/bdb/products/vialimages/packaging-images/black-vial-images/Blue%20Cap%20-%20Blue%20Label%20(Transparent).png?imwidth=320)
The M5E2 monoclonal antibody specifically binds to CD14, a 53–55 kDa glycosylphosphatidylinositol (GPI)-anchored single chain glycoprotein expressed at high levels on monocytes. Additionally, the anti-CD14 antibody reacts with interfollicular macrophages, reticular dendritic cells, and some Langerhans cells. CD14 has been identified as a high affinity cell-surface receptor for complexes of lipopolysaccharide (LPS) and serum LPS-binding protein, LPB.
![altImg](/content/dam/bdb/products/vialimages/packaging-images/formatimages/pe_cy7_spectrum_guide.jpg)
Development References (3)
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Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182.
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McMichael AJ. A.J. McMichael .. et al., ed. Leucocyte typing III : white cell differentiation antigens. Oxford New York: Oxford University Press; 1987:1-1050.
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Wright SD, Ramos RA, Tobias PS, Ulevitch RJ, Mathison JC. CD14, a receptor for complexes of lipopolysaccharide (LPS) and LPS binding protein. Science. 1990; 249(4975):1431-1433. (Biology). View Reference
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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