Preparation And Storage
The FITC and PE conjugates are each supplied in 1.0 mL of phosphate buffered saline containing gelatin and 0.1% sodium azide. Each lot is bottled at titer as determined by immunofluorescence staining of lymphocytes in peripheral blood. Please refer to the vial label for antibody concentration. The vial should be stored at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.
The CD26 antibody, clone L272, is derived from hybridization of mouse Sp2/0 cells with spleen cells from BALB/c mice immunized with E1αPGF/JY cells.
CD26 recognizes a 120-kilodalton (kDa) antigen that is identical to the enzyme dipeptidyl peptidase (DPP), a serine protease. It is associated with the binding of the TAT transactivating protein of the human immunodeficiency virus (HIV). CD26 and CD45 act in a co-stimulatory fashion on T lymphocytes.
Development References (9)
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Blazquez M, Maduceno J, Gonzalez R, et al. Selective decrease of CD26 expression in T cells from HIV-1 infected individuals. J Immunol. 1992; 149(9):3073-3077. (Biology).
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Duke-Cohan JS, Morimoto C, Schlossman SF. Targeting of an activated T-cell subset using a bispecific antibody-toxin conjugate directed against CD4 and CD26. Blood. 1993; 82:2224-2234. (Biology).
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Gutheil WG, Subramanyam M, Flentke GR, et al. Human immunodeficiency virus 1 Tat binds to dipeptidyl aminopeptidase IV (CD26): a possible mechanism for Tat's immunosuppressive activity.. Proc Natl Acad Sci USA. 1994; 91(14):6594-8. (Biology). View Reference
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Morimoto C, Kameoka J, Tanaka T, Schlossman S. Overview of CD26. In: Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995:1105-1114.
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Muñoz E, Blazquez MV, Madueño JA, Rubio G, Peña J. CD26 induces T-cell proliferation by tyrosine protein phosphorylation.. Immunology. 1992; 77(1):43-50. (Biology). View Reference
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Plana M, Font J, Viñas O, Martorell J, Ingelmo M, Vives J. Responsiveness of T lymphocytes from systemic lupus erythematosus to signals provided through CD26 antigen.. Clin Immunol Immunopathol. 1994; 72(2):227-32. (Biology). View Reference
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Stein H, Schwarting R, Niedobitek G. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:412-415.
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Ulmer AJ, Mattern T, Flad HD. Expression of CD26 (dipeptidyl peptidase IV) on memory and naive T lymphocytes.. Scand J Immunol. 1992; 35(5):551-9. (Biology). View Reference
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Vanham G, Kestens L, De Meester I, et al. Decreased expression of the memory marker CD26 on both CD4+ and CD8+ T lymphocytes of HIV-infected subjects.. J Acquir Immune Defic Syndr. 1993; 6(7):749-57. (Biology). View Reference
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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