APC Mouse Anti-Human HER-2/neu
Clone NEU 24.7 (RUO (GMP))
- Alternative Name Neu, Her-2
- Vol. Per Test 5 μL
- Isotype Mouse BALB/c IgG1
- Reactivity Human (QC Testing)
Flow cytometry (Routinely Tested)
- Immunogen SKBR3 human breast carcinoma cells
- Storage Buffer Phosphate buffered saline with gelatin and 0.1% sodium azide.
- Regulatory Status RUO (GMP)
Regulatory Status Legend
Anti–HER-2/neu, clone Neu 24.7, is derived from the fusion of NSO myeloma cells with spleen cells from BALB/c mice injected with living SKBR3 human breast carcinoma cells.
Anti–HER-2/neu recognizes a single protein of 185 kdaltons (kDa) from HER-2 transfected cells.
Allophycocyanin (APC), is an accessory photosynthetic pigment found in blue-green algae. Its molecular weight is approximately 105 kDa. APC has six phycocyanobilin chromophores per molecule, which makes it a very bright fluorochrome that is highly suitable for flow cytometry applications. Due to nearly identical excitation and emission properties but different spillover characteristics, APC and Alexa Fluor® 647 cannot be used simultaneously
Preparation and Storage
The FITC and PE conjugates are each supplied in 1.0 mL of PBS. The APC conjugate is supplied in 0.5 mL. Please refer to the vial label for antibody concentration. PBS contains gelatin and 0.1% sodium azide. Vials should be stored at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.