APC Mouse Anti-Human EpCAM
Clone EBA-1 (RUO (GMP))
- Alternative Name EpCAM
- Concentration 12 μg/mL
- Vol. Per Test 5 μL
- Isotype Mouse BALB/c IgG1, λ
- Reactivity Human (QC Testing)
Flow cytometry (Routinely Tested)
- Immunogen Breast carcinoma–associated mucin BCA-225
- Entrez Gene ID 4072
- Storage Buffer Phosphate buffered saline with gelatin and 0.1% sodium azide.
- Regulatory Status RUO (GMP)
Regulatory Status Legend
Anti-EpCAM, clone EBA-1, is derived from the fusion of SP2 myeloma cells with spleen cells from BALB/c mice immunized with macromolecular aggregates containing breast carcinoma–associated mucin BCA-225.
Anti-EpCAM is directed against a human epithelial adhesion molecule (EpCAM) that has been identified as a ~40-kilodalton (kd) protein in Western blotting. This antigen is also known as epithelial surface antigen (ESA) and epithelial glycoprotein 2 (EGP-2).
Allophycocyanin (APC), is an accessory photosynthetic pigment found in blue-green algae. Its molecular weight is approximately 105 kDa. APC has six phycocyanobilin chromophores per molecule, which makes it a very bright fluorochrome that is highly suitable for flow cytometry applications. Due to nearly identical excitation and emission properties but different spillover characteristics, APC and Alexa Fluor® 647 cannot be used simultaneously
Preparation and Storage
The FITC conjugate is supplied as 3 µg in 1.0 mL (3 µg/mL) of phosphate-buffered saline (PBS). The PE conjugate is supplied as 3 µg in 1.0 mL (3 µg/mL) of PBS. The PerCP-Cy5.5 conjugate is supplied as 2 µg in 1.0 mL (2 µg/mL) of PBS. The APC conjugate is supplied as 6 µg in 0.5 mL (12 µg/mL) of PBS. PBS contains gelatin and 0.1% sodium azide. Vials should be stored at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.