APC Mouse Anti-Human CD338

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BD Pharmingen™ APC Mouse Anti-Human CD338

Name: APC Mouse Anti-Human CD338
Brand: BD Pharmingen™
SKU: 561451

Clone 5D3/CD338

(RUO)

Flow cytometric analysis of CD338 expression on human placenta epithelial choriocarcionma. JEG-3 (ATCC Cat. No. HTB-36) cells were stained with either APC Mouse Anti-Human CD338 antibody (Cat. No. 561451, solid line histogram) or an APC mIgG2b, κ isotype control (Cat. No. 555745; dashed line histogram). Flow cytometric fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry was performed using a BD™ LSR II flow cytometry system.

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Product Details

Brand: BD Pharmingen™

Alternative Name: ABCG2; ABCP; BCRP; BMDP; MXR1; ABC15; BCRP1; CDw338; EST157481; MGC102821

Reactivity: Human (QC Testing)

Isotype: Mouse IgG2b, κ

Immunogen: Human ABCG2-transfected Mouse cells

Application: Flow cytometry (Routinely Tested)

Vol. Per Test: 5 µl

Entrez Gene ID: 9429

RRID: AB_10715578

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed.

Product Notices

This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
An isotype control should be used at the same concentration as the antibody of interest.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

Companion Products

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

561451 Rev. 2

Antibody Details

5D3/CD338

The 5D3/CD338 monoclonal antibody specifically binds to an epitope of ABCG2 (BCRP1), a multi-drug resistance protein that is a member of ATP binding cassette (ABC) transporters. It is highly expressed on primitive stem cells as identified by the "side-population" (SP) phenotype. This SP phenotype is based on the efflux of fluorescent dyes such as Rhodamine 123 and Hoechst 33342. The expression of ABCG2 appears to be highly conserved as it has been identified in various species. Studies show that highly purified murine stem cells express BCRP1 mRNA and this expression declines sharply as the stem cells express CD34. The highest levels of BCRP1 mRNA expression have been seen in KDR+ human stem cells. ABCG2/BCRP1 was clustered as CD338 in the HLDA8 workshop.

561451 Rev. 2

Format Details

APC

Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: APC

Excitation Source: Red 627-640 nm

Excitation Max: 651 nm

Emission Max: 660 nm

561451 Rev.2

Citations & References

Development References (4)

Bunting KD. ABC transporters as phenotypic markers and functional regulators of stem cells. Stem Cells. 2002; 20(1):11-20. (Biology). View Reference
Ozvegy-Laczka C, Laczkó R, Hegedus, et al. Interaction with the 5D3 monoclonal antibody is regulated by intramolecular rearrangements but not by covalent dimer formation of the human ABCG2 multidrug transporter. J Biol Chem. 2008; 283(38):26059-26070. (Clone-specific: Flow cytometry). View Reference
Zhou S, Schuetz JD, Bunting KD, et al. The ABC transporter Bcrp1/ABCG2 is expressed in a wide variety of stem cells and is a molecular determinant of the side-population phenotype. Nat Med. 2001; 7(9):1028-1034. (Clone-specific). View Reference
Zola H, Swart B, Nicholson I, et al . CD molecules 2005: human cell differentiation molecules . Blood. 2005; 106(9):3123-3126. (Clone-specific). View Reference

View All (4)

561451 Rev. 2

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.