AmCyan Mouse Anti-Human CD45
Clone 2D1 (RUO (GMP))
- Concentration 100 μg/mL
- Vol. Per Test 5 μL
- Isotype Mouse IgG1, κ
- Reactivity Human (QC Testing)
Flow cytometry (Routinely Tested)
- Storage Buffer Phosphate buffered saline with BSA and 0.1% sodium azide.
- Regulatory Status RUO (GMP)
Regulatory Status Legend
CD45 clone 2D1 is derived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human peripheral blood mononuclear cells (PBMCs).
The CD45 antibody (anti–HLe-1) recognizes members of the T200 family of human leucocyte antigens with molecular mass of 180 to 220 kilodaltons (kDa).
AmCyan is a 108-kDa protein derived from Anemonia majano. With an excitation peak of 457 nm and an emission peak of 491 nm, it can be used on violet laser–equipped flow cytometers in combination with BD Horizon BV421, BD Horizon V450, or Pacific Blue™, but not BD Horizon™ V500.
Preparation and Storage
Store vials at 2°C to 8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.