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      Biotin Mouse Anti-Rat IgG2c
      Product Details
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      BD Pharmingen™
      Rat (QC Testing)
      Mouse BALB/c IgG1, κ
      Pooled rat IgG2c
      ELISA (Routinely Tested), Flow cytometry, Immunohistochemistry-frozen, Intracellular staining (flow cytometry) (Tested During Development)
      0.5 mg/ml
      AB_395128
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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      Recommended Assay Procedures

      ELISA: For the rat IgG2c sandwich ELISA, biotinylated A92-1 mAb is optimal for detection when used with purified A92-3 mAb (Cat. no. 553910) for capture and purified rat IgG2c κ , Cat. No. 553982,  for the protein standard.

      Other applications: Biotinylated A92-1 mAb may be used as a secondary reagent in immunofluorescent surface staining. Other reported applications include flow cytometric detection of intracytoplasmic IgG2c and immunohistochemical staining of acetone-fixed frozen sections.

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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      553909 Rev. 10
      Antibody Details
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      A92-1

      The A92-1 antibody reacts specifically with rat IgG2c. It does not react with other Ig isotypes.

      553909 Rev. 10
      Format Details
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      Biotin
      Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
      Biotin
      553909 Rev.10

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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