Purified Mouse Anti-NeuroD1
Clone R8-294 (RUO)
- Brand BD Pharmingen™
- Alternative Name Class A basic helix-loop-helix protein 3, bHLHa3, BETA2, NeuroD
- Concentration 0.5 mg/ml
- Isotype Mouse IgG1, κ
- Reactivity Human (QC Testing) Mouse (Tested in Development)
Western blot (Routinely Tested)
Bioimaging, Immunofluorescence, Intracellular staining (flow cytometry) (Tested During Development)
- Immunogen Human NeuroD1 Recombinant Protein
- Storage Buffer Aqueous buffered solution containing ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
The R8-294 monoclonal antibody specifically binds to Neurogenic differentiation factor 1 (NeuroD1). NeuroD1 is a basic helix-loop-helix (bHLH) transcription factor that is also known as BHLHA3, Beta-cell E-box transactivator 2, BETA2 and NeuroD. NeuroD1 interacts with other bHLH proteins and binds the insulin E-box sequence to regulate pancreatic islet cell development. It also plays a key role in central nervous system (CNS) and sensory nervous system neuron development. Specifically, it is required for the terminal differentiation of neurons during late stages of neurogenesis in the CNS (cerebral cortex, hippocampus, and cerebellum) and the sensory nervous system (eye, inner ear, and olfactory system). During antibody development, the purified R8-294 monoclonal antibody was found to detect NeuroD1 by western blot and indirect immunofluorescent staining followed by flow cytometric analysis or imaging analysis.
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Preparation and Storage
Store undiluted at 4°C.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
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- Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.