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Purified Mouse Anti-Human TNF MAb11 RUO

Purified Mouse Anti-Human TNF 

Clone  MAb11   (RUO)

  • Brand BD Pharmingen™
  • Alternative Name Tumor necrosis factor alpha; TNF-a; TNF-α; TNFSF2; Cachectin
  • Concentration 0.5 mg/ml
  • Isotype Mouse IgG1, κ
  • Reactivity Human (QC Testing)  Rhesus, Baboon, Cynomolgus (Tested in Development) 
  • Application Intracellular block/flow cytometry (Routinely Tested) 
  • Immunogen Recombinant Human TNF
  • Storage Buffer Aqueous buffered solution containing ≤0.09% sodium azide.
  • Regulatory Status RUO
Product Details Recommended Assay References

Description

The MAb11 monoclonal antibody specifically binds to human tumor necrosis factor (TNF, also known as TNF-α) protein. TNF is an efficient juxtacrine, paracrine and endocrine mediator of inflammatory and immune functions. It regulates the growth and differentiation of a variety of cell types. TNF is cytotoxic for transformed cells when in conjunction with IFN-γ. It is secreted by activated monocytes/macrophages and other cells such as B cells, T cells and fibroblasts. The immunogen used to generate the MAb11 hybridoma was recombinant human TNF. The MAb11 antibody has been reported to crossreact with Rhesus Macaque TNF.

Format
  • Format Purified

Other Formats →

Suggested Companion Products

PE Mouse Anti-Human TNF MAb11 RUO
0.1 mg Cat No: 554513

FITC Mouse Anti-Human TNF MAb11 RUO
0.1 mg Cat No: 554512

BD Cytofix/Cytoperm Plus Kit (with BD GolgiStop) RUO
250 Tests Cat No: 554715

HiCK-1 Human Cytokine Positive Control Cells RUO
1 mL Cat No: 555061

Stain Buffer (FBS) RUO
500 mL Cat No: 554656

Stain Buffer (BSA) RUO
500 mL Cat No: 554657

Purified Mouse Anti-Human TNF MAb11 RUO
0.1 mg Cat No: 558882

PE-Cy™5 Mouse Anti-Human CD3 UCHT1 (also known as UCHT-1; UCHT 1) RUO
100 Tests Cat No: 555334

Recombinant Human TNF RUO
10 µg Cat No: 554618

Resources & Tools
 Spectrum Viewer  Download TDS  Regulatory Document Website
Preparation and Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
  5. Species testing during development may have been performed with a different format of the same clone. Selected applications have been tested for cross-reactivity.
  6. Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.


Blocking Control for Intracellular Staining: The purified MAb11 antibody can be used as a blocking control to demonstrate specificity of TNF staining by PE-MAb11 (Cat. No. 554513) or FITC-MAb11 antibody (Cat. No. 554512). To perform this control, the fixed/permeabilized cells (~1 million) can be incubated with 1 -10 µg of unlabeled MAb11 antibody (Cat. No. 554510) for 20 minutes at 4°C, prior to staining with PE-MAb11 or FITC-MAb11 antibody (e.g., 0.1 - 0.5 µg mAb/1 million cells). The intracellular cytokine staining technique and use of blocking controls are described in detail by C. Prussin and D. Metcalfe. For specific methodology, please visit the protocols section under "Cytokines (Intracellular Staining)" or "Intracellular Flow" at our website, http://www.bdbiosciences.com/us/s/resources.


  1. Danis VA, Franic GM, Rathjen DA, Brooks PM. Effects of granulocyte-macrophage colony-stimulating factor (GM-CSF), IL-2, interferon-gamma (IFN-gamma), tumour necrosis factor-alpha (TNF-alpha) and IL-6 on the production of immunoreactive IL-1 and TNF-alpha by human monocytes. Clin Exp Immunol. 1991; 85(1):143-150. View reference

  2. Jason J, Larned J. Single-cell cytokine profiles in normal humans: comparison of flow cytometric reagents and stimulation protocols. J Immunol Methods. 1997; 207(1):13-22. View reference

  3. Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. View reference

  4. Rathjen DA, Cowan K, Furphy LJ, Aston R. Antigenic structure of human tumour necrosis factor: recognition of distinct regions of TNF alpha by different tumour cell receptors. Mol Immunol. 1991; 28(1-2):79-86. View reference

  5. Sander B, Andersson J, Andersson U. Assessment of cytokines by immunofluorescence and the paraformaldehyde-saponin procedure. Immunol Rev. 1991; 119:65-93. View reference

  6. Sopper S, Stahl-Hennig C, Demuth M, Johnston IC, Dorries R, ter Meulen V. Lymphocyte subsets and expression of differentiation markers in blood and lymphoid organs of rhesus monkeys. Cytometry. 1997; 29(4):351-362. View reference

  7. Verdier F, Aujoulat M, Condevaux F, Descotes J. Determination of lymphocyte subsets and cytokine levels in cynomolgus monkeys. Toxicology. 1995; 105(1):81-90. View reference

554510 Rev. 2
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