APC Rat Anti-Human IL-2
Clone MQ1-17H12 (RUO)
- Brand BD Pharmingen™
- Vol. Per Test 20 µl
- Isotype Rat IgG2a, κ
- Reactivity Rhesus, Cynomolgus, Baboon (QC Testing)
Intracellular staining (flow cytometry) (Routinely Tested)
- Immunogen Human IL-2 Recombinant Protein
- Storage Buffer Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
Clone MQ1-17H12 reacts with the human form of interleukin-2 (IL-2) detectable in the cytoplasm of a subset of activated peripheral blood T lymphocytes, following treatment with monensin or brefeldin A. Clone MQ1-17H12 also cross-reacts with a cytoplasmic component of peripheral blood CD3+ lymphocytes of baboon, and both rhesus and cynomolgus macaque monkeys following six-hour treatment with phorbol myristic acetate (PMA) and Ca++ ionophore (A23187) in the presence of monensin. While the frequency of CD3+ non-human primate cells reacting with MQ1-17H12 following PMA + Ca++ ionophore activation is similar to that observed with normal human donor CD3+ lymphocytes, the fluorescence intensity is characteristically weaker.
Allophycocyanin (APC), is an accessory photosynthetic pigment found in blue-green algae. Its molecular weight is approximately 105 kDa. APC has six phycocyanobilin chromophores per molecule, which makes it a very bright fluorochrome that is highly suitable for flow cytometry applications. Due to nearly identical excitation and emission properties but different spillover characteristics, APC and Alexa Fluor® 647 cannot be used simultaneously
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Preparation and Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.