APC Mouse Anti-Human CD208
Clone I10-1112 (RUO)
- Brand BD Pharmingen™
- Alternative Name DC-LAMP
- Vol. Per Test 20 µl
- Isotype Mouse IgG1, κ
- Reactivity Human (QC Testing)
Intracellular staining (flow cytometry) (Routinely Tested)
- Storage Buffer Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
Reacts with CD208, also known as DC-LAMP or lysosome associate membrane protein. DC-LAMP is an intracellular molecule, of approximately 70 - 90 kDa, induced during dendritic cell (DC) maturation. It is undetected in immature DCs, but it is sharply expressed upon activation with LPS, TNFa, or CD154 in DCs from skin, tonsil, cultures of CD34+ cells in presence of GM-CSF and TNFa, and on monocyte cultures in the presence of GM-CSF and IL-4. Reports describe the transient expression of CD208 in the MHC class II compartment before the translocation of the MHC II molecules to the cell surface. It has been suggested that the biological role of DCLAMP could be its participation in peptide loading onto MHC class II.
Allophycocyanin (APC), is an accessory photosynthetic pigment found in blue-green algae. Its molecular weight is approximately 105 kDa. APC has six phycocyanobilin chromophores per molecule, which makes it a very bright fluorochrome that is highly suitable for flow cytometry applications. Due to nearly identical excitation and emission properties but different spillover characteristics, APC and Alexa Fluor® 647 cannot be used simultaneously
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Preparation and Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.