APC Mouse Anti-Human CD208

BD Pharmingen™ APC Mouse Anti-Human CD208

Name: APC Mouse Anti-Human CD208
Brand: BD Pharmingen™
SKU: 558168

Clone I10-1112

(RUO)

Profile of CD208, DC-LAMP (I10-1112) reactivity on fixed and permeablized monocyte- derived cultured dendritic cells with 1% Paraformaldehyde and 70% Ethanol Fixation analyzed by flow cytometry

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Product Details

Brand: BD Pharmingen™

Alternative Name: DC-LAMP

Reactivity: Human (QC Testing)

Isotype: Mouse IgG1, κ

Application: Intracellular staining (flow cytometry) (Routinely Tested)

Vol. Per Test: 20 µl

RRID: AB_647160

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Companion Products

APC Mouse IgG1, κ Isotype Control RUO

Size 100 Tests Cat No. 555751

558168 Rev. 2

Antibody Details

I10-1112

Reacts with CD208, also known as DC-LAMP or lysosome associate membrane protein. DC-LAMP is an intracellular molecule, of approximately 70 - 90 kDa, induced during dendritic cell (DC) maturation. It is undetected in immature DCs, but it is sharply expressed upon activation with LPS, TNFa, or CD154 in DCs from skin, tonsil, cultures of CD34+ cells in presence of GM-CSF and TNFa, and on monocyte cultures in the presence of GM-CSF and IL-4. Reports describe the transient expression of CD208 in the MHC class II compartment before the translocation of the MHC II molecules to the cell surface. It has been suggested that the biological role of DCLAMP could be its participation in peptide loading onto MHC class II.

558168 Rev. 2

Format Details

APC

Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: APC

Excitation Source: Red 627-640 nm

Excitation Max: 651 nm

Emission Max: 660 nm

558168 Rev.2

Citations & References

Development References (2)

Banchereau J, Briere F, Caux C. Immunobiology of dendritic cells. Annu Rev Immunol. 2000; 18:767-811. (Biology). View Reference
de Saint-Vis B, Vincent J, Vandenabeele S. de Saint-Vis B, Vincent J, Vandenabeele S. Immunity. 1998; 9(3):325-336. (Biology). View Reference

558168 Rev. 2

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.