Analysis of Sox1 in H9 Embryonic Stem (ES) cells (left panel) and H9-derived Neural Stem Cells (NSC) (right panel). H9 human ES cells (WiCell, Madison, WI) and H9-derived NSC were harvested with BD™ Accutase™ Cell Detachment Solution (Cat. No. 561527), fixed in BD Cytofix™ Fixation Buffer (Cat. No. 554655), and permeabilized with BD Phosflow™ Perm Buffer III (Cat. No.558050). Cells were stained with matching concentrations of either Alexa Fluor® 647 Mouse Anti-Human Sox1 antibody (solid lines) or Alexa Fluor® 647 Mouse IgG1, κ isotype control (dashed lines, Cat. No.557783 ). Histograms were derived from gated events based on light scattering characteristics for the ES and NSC respectively. Flow cytometry was performed on a BD™ LSR II flow cytometry system.
Analysis of Sox1 in H9 Embryonic Stem (ES) cells (left panel) and H9-derived Neural Stem Cells (NSC) (right panel). H9 human ES cells (WiCell, Madison, WI) and H9-derived NSC were harvested with BD™ Accutase™ Cell Detachment Solution (Cat. No. 561527), fixed in BD Cytofix™ Fixation Buffer (Cat. No. 554655), and permeabilized with BD Phosflow™ Perm Buffer III (Cat. No.558050). Cells were stained with matching concentrations of either Alexa Fluor® 647 Mouse Anti-Human Sox1 antibody (solid lines) or Alexa Fluor® 647 Mouse IgG1, κ isotype control (dashed lines, Cat. No.557783 ). Histograms were derived from gated events based on light scattering characteristics for the ES and NSC respectively. Flow cytometry was performed on a BD™ LSR II flow cytometry system.
Product Details
BD Pharmingen™
Transcription factor SOX-1; SRY (sex determining region Y)-box 1
Human (QC Testing)
Mouse IgG1, κ
Human Sox1 Peptide
Intracellular staining (flow cytometry) (Routinely Tested)
5 µl
AB_11154034
Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- An isotype control should be used at the same concentration as the antibody of interest.
- mTESR™1 is a trademark of StemCell Technologies.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Fixation Buffer RUO
Size
100 mL
Cat No.
554655
Perm Buffer III RUO
Size
125 mL
Cat No.
558050
Alexa Fluor® 647 Mouse IgG1 κ Isotype control RUO
Size
50 Tests
Cat No.
557783
Accutase™ Cell Detachment Solution RUO
Size
100 mL
Cat No.
561527
562224 Rev. 1
Antibody Details
N23-844
The N23-844 monoclonal antibody reacts with human Sox1, a member of the SOX [SRY (sex determining region Y)-HMG-box] family of transcription factors. The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins. It is one of the earliest transcription factors to be expressed in ectodermal cells committed to the neural fate. Sox1 is expressed in both embryonic and somatic neural stem and progenitor cells, and it is down regulated during neuronal differentiation in many neuronal subtypes.
562224 Rev. 1
Format Details
Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
562224 Rev.1
Citations & References
Development References (4)
-
Kan L, Israsena N, Zhang Z, et al. Sox1 acts through multiple independent pathways to promote neurogenesis. Dev Biol. 2004; 15;26(2):580-594. (Biology). View Reference
-
Malas S, Duthie SM, Mohri F, Lovell-Badge R, Episkopou V. Cloning and mapping of the human SOX1: a highly conserved gene expressed in the developing brain. Mamm Genome. 1997; 8(11):866-868. (Biology). View Reference
-
Pevny LH, Sockanathan S, Placzek M, Lovell-Badge R. A role for SOX1 in neural determination. Development. 1998; 125(10):1967-1978. (Biology). View Reference
-
Wilson M, Koopman P. Matching SOX: partner proteins and co-factors of the SOX family of transcriptional regulators. Curr Opin Genet Dev. 2002; 12(4):441-446. (Biology). View Reference
562224 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
