APC Mouse Anti-Human CD10
Clone HI10a (RUO (GMP))
- Concentration 25 μg/mL
- Vol. Per Test 5 μL
- Isotype Mouse BALB/c IgG1, κ
- Reactivity Human (QC Testing)
Flow cytometry (Routinely Tested)
- Immunogen Acute CALLA Leukemia Blast Cells
- Workshop No. V CD10.7
- Entrez Gene ID 4311
- Storage Buffer Phosphate buffered saline with gelatin and 0.1% sodium azide.
- Regulatory Status RUO (GMP)
Regulatory Status Legend
The CD10 antibody, clone HI10a, is derived from the hybridization of mouse P3-63-Ag8.653 myeloma cells with spleen cells of BALB/c mice immunized with blasts from a patient with acute CALLA leukemia.
The CD10 antibody recognizes a 100-kilodalton (kDa) type II transmembrane, glycosylated, zinc-containing metalloprotease. The CD10 antigen is also known as common acute lymphoblastic leukemia antigen (CALLA), neutral endopeptidase (NEP), gp100, and enkephalinase.
Allophycocyanin (APC), is an accessory photosynthetic pigment found in blue-green algae. Its molecular weight is approximately 105 kDa. APC has six phycocyanobilin chromophores per molecule, which makes it a very bright fluorochrome that is highly suitable for flow cytometry applications. Due to nearly identical excitation and emission properties but different spillover characteristics, APC and Alexa Fluor® 647 cannot be used simultaneously
Preparation and Storage
Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.